A new behavioural apparatus to reduce animal numbers in multiple types of spontaneous object recognition paradigms in rats
A new behavioural apparatus to reduce animal numbers in multiple types of spontaneous object recognition paradigms in rats
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DOI：
10.1016/j.jneumeth.2012.08.006
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发表时间：
2012-10-15
影响因子：
3
通讯作者：
Easton, A.
中科院分区：
文献类型：
--
作者：
Ameen-Ali, K. E.;Eacott, M. J.;Easton, A.
标准的物体识别程序通过动物自发探索新物体或物体与其环境的其他方面的新配置来评估动物的记忆。这类任务广泛用于记忆研究，也用于制药公司筛选新药治疗。然而，这些任务中的行为可能受到新奇感以外的影响，例如来自处理的压力，这些压力随后会影响性能。这种额外的实验差异意味着需要大量的动物来维持权力。此外，数据的积累是耗时的，因为动物通常每天只进行一次试验。本研究旨在探讨如何有效地识别记忆可以测试与一个新的连续试验装置，允许在一个会话中的多次试验，并减少处理压力，通过结合延迟非匹配到样本和自发的物体识别任务的功能。在该装置中，Lister hooded大鼠在对象识别任务（实验1和2）和对象定位任务（实验3）和对象上下文记忆（实验4）中显示出显着高于机会水平的性能，每个实验组只有5只动物或更少的数据。研究结果表明，这些结果与文献中先前报告的结果相当，并且保持了统计功效，同时使用的动物数量不到自发识别范式中通常使用的动物数量的三分之一。总的来说，这些结果突出了连续试验装置在减少识别记忆任务中使用的动物数量方面的潜在益处。(C)2012爱思唯尔有限公司版权所有。
Standard object recognition procedures assess animals' memory through their spontaneous exploration of novel objects or novel configurations of objects with other aspects of their environment. Such tasks are widely used in memory research, but also in pharmaceutical companies screening new drug treatments. However, behaviour in these tasks may be driven by influences other than novelty such as stress from handling which can subsequently influence performance. This extra-experimental variance means that large numbers of animals are required to maintain power. In addition, accumulation of data is time consuming as animals typically perform only one trial per day. The present study aimed to explore how effectively recognition memory could be tested with a new continual trials apparatus which allows for multiple trials within a session and reduced handling stress through combining features of delayed nonmatching-to-sample and spontaneous object recognition tasks. In this apparatus Lister hooded rats displayed performance significantly above chance levels in object recognition tasks (Experiments 1 and 2) and in tasks of object-location (Experiment 3) and object-in-context memory (Experiment 4) with data from only five animals or fewer per experimental group. The findings indicated that the results were comparable to those of previous reports in the literature and maintained statistical power whilst using less than a third of the number of animals typically used in spontaneous recognition paradigms. Overall, the results highlight the potential benefit of the continual trials apparatus to reduce the number of animals used in recognition memory tasks. (C) 2012 Elsevier B.V. All rights reserved.