Use of monoclonal anti-mouse immunoglobulin to detect mouse antibodies.
Use of monoclonal anti-mouse immunoglobulin to detect mouse antibodies.
复制标题
使用单克隆抗小鼠免疫球蛋白检测小鼠抗体。
DOI：
10.1089/hyb.1.1981.1.5
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Scharff,MD
中科院分区：
文献类型：
--
作者：
Yelton,DE;Desaymard,C;Scharff,MD
已生成对小鼠κ轻链和小鼠γ重链具有特异性的大鼠单克隆抗体。这些大鼠单克隆抗体已用35S甲硫氨酸生物合成标记。将游离标记从培养基中透析出来，不经进一步纯化，将含有放射性单克隆抗体的培养基用于放射免疫测定，以筛选免疫动物和杂交瘤的血清中IgG类的特异性小鼠抗体。
Rat monoclonal antibodies specific for mouse kappa light chains and mouse γ heavy chains have been generated. These rat monoclonal antibodies have been biosynthetically labelled with35S methionine. The free label was dialyzed from the medium and, without further purification, the medium containing the radioactive monoclonal antibody was used in a radioimmunoassay to screen the sera of the immunized animals and hybridomas for specific mouse antibodies of the IgG class.