A novel approach to the simultaneous extraction and non-targeted analysis of the small molecules metabolome and lipidome using 96-well solid phase extraction plates with column-switching technology
A novel approach to the simultaneous extraction and non-targeted analysis of the small molecules metabolome and lipidome using 96-well solid phase extraction plates with column-switching technology
复制标题
使用采用柱切换技术的 96 孔固相萃取板同时提取和非靶向分析小分子代谢组和脂质组的新方法
DOI：
10.1016/j.chroma.2015.07.048
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发表时间：
2015-08-28
影响因子：
4.1
通讯作者：
Zhang, Yanjun
中科院分区：
文献类型：
--
作者：
Li, Yubo;Zhang, Zhenzhu;Zhang, Yanjun
本研究将96孔板固相萃取（SPE）与柱切换技术相结合，构建了基于超高效液相色谱四极杆飞行时间质谱的小分子代谢组和脂质组的同时提取和非靶向分析的快速高通量方法。本研究首先研究了小分子代谢组和脂质组的色谱柱和分析条件，分别由 HSS T3 和 BEH C18 色谱柱分离。接下来，进一步优化了SPE的负载能力和驱动持续时间。随后，将SPE和柱切换结合起来，对生物样品进行快速、全面的分析。实验结果表明，新的分析方法具有良好的精密度并保持了样品的稳定性（RSD < 15%）。然后该方法令人满意地应用于更广泛地分析小分子代谢组和脂质组以测试通量。由此产生的方法代表了一种新的生物样品分析方法，也是代谢组学和脂质组学研究的非常有用的工具。 (C) 2015 Elsevier B.V. 保留所有权利。
This study combines solid phase extraction (SPE) using 96-well plates with column-switching technology to construct a rapid and high-throughput method for the simultaneous extraction and non-targeted analysis of small molecules metabolome and lipidome based on ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry. This study first investigated the columns and analytical conditions for small molecules metabolome and lipidome, separated by an HSS T3 and BEH C18 columns, respectively. Next, the loading capacity and actuation duration of SPE were further optimized. Subsequently, SPE and column switching were used together to rapidly and comprehensively analyze the biological samples. The experimental results showed that the new analytical procedure had good precision and maintained sample stability (RSD < 15%). The method was then satisfactorily applied to more widely analyze the small molecules metabolome and lipidome to test the throughput. The resulting method represents a new analytical approach for biological samples, and a highly useful tool for researches in metabolomics and lipidomics. (C) 2015 Elsevier B.V. All rights reserved.