Time-course of changes in neuronal activity markers following iTBS-TMS of the rat neocortex
Time-course of changes in neuronal activity markers following iTBS-TMS of the rat neocortex
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DOI：
10.1016/j.neulet.2013.01.003
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发表时间：
2013-03-01
影响因子：
2.5
通讯作者：
Funke, Klaus
中科院分区：
文献类型：
--
作者：
Hoppenrath, Kathrin;Funke, Klaus
在大鼠模型的经颅磁刺激，我们最近可以表明，间歇性theta-突发刺激（iTBS）影响的立即早期基因产物c-Fos和zif 268，以及两个谷氨酸脱羧酶亚型GAD 65和GAD 67和钙结合蛋白（CB）和小清蛋白（PV），被称为兴奋和抑制活性的标志物的新皮层表达。我们现在更详细地分析了在由600个刺激组成的iTBS的单个阻断后10、20、40、80和160分钟这些蛋白质表达变化的时间过程。c-Fos、zif 268和GAD 65的初始增加（20分钟）发出兴奋性和抑制性神经元的瞬时激活的信号，此后首先是抑制性神经元的活性标记物的降低（GAD 67、PV、CB：20-80分钟），然后是c-Fos和GAD 65表达的晚期降低（160分钟）。结果表明，一种iTBS阻断可具有至少两个不同阶段的后效，早期阶段具有增加的神经元活性（c-Fos，zif 268），但也具有增加的GABA释放（GAD 65）的可能性，随后是兴奋性和抑制性系统中减少的神经元活性的晚期阶段（>40分钟），其可指示减少的兴奋性的状态。(C)2013爱思唯尔爱尔兰有限公司版权所有。
In a rat model of transcranial magnetic stimulation we could recently show that intermittent theta-burst stimulation (iTBS) affects the neocortical expression of the immediate early gene products c-Fos and zif268 as well as that of the two glutamic acid decarboxylase isoforms GAD65 and GAD67 and that of the calcium-binding proteins calbindin (CB) and parvalbumin (PV), known as markers of excitatory and inhibitory activity. We now analyzed in more detail the time course of changes in the expression of these proteins at 10, 20, 40, 80 and 160 min following a single block of iTBS consisting of 600 stimuli. Initial increase in c-Fos, zif268 and GAD 65 (20 min) signals transient activation of excitatory and inhibitory neurons, thereafter first followed by a decrease in markers of activity of inhibitory neurons (GAD67, PV, CB: 20-80 min) and then by a late decrease in c-Fos and GAD65 expression (160 min). The results demonstrate that one iTBS block may have an after-effect of at least two different phases, an early phase with increased neuronal activity (c-Fos, zif268) but also the likelihood of increased GABA-release (GAD65), followed by a late phase (>40 min) of reduced neuronal activity in excitatory and inhibitory systems which may indicate a state of reduced excitability. (C) 2013 Elsevier Ireland Ltd. All rights reserved.