Influx of neutrophils into the airway lumen at 4 h after segmental allergen challenge in asthma
Influx of neutrophils into the airway lumen at 4 h after segmental allergen challenge in asthma
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DOI：
10.1159/000024174
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发表时间：
1999-05-01
影响因子：
2.8
通讯作者：
van der Zee, JS
中科院分区：
文献类型：
--
作者：
Nocker, RET;Out, TA;van der Zee, JS
背景：节段性过敏原激发是研究哮喘气道炎症反应的有力工具。关于过敏原攻击后 5 分钟和 4 小时的早期事件，关于细胞流入和趋化因子白细胞介素 8 (IL-8) 的信息很少。方法：7 名轻度至中度过敏性哮喘患者（AA 组）、5 名过敏性非哮喘患者（ANA 组）和 5 名非过敏对照者接受分段过敏原激发，过敏原剂量基于皮肤反应性。在攻击前、攻击后 5 分钟和 4 小时获取支气管肺泡损伤 (BAL) 样本，并分析细胞数量和差异计数、嗜酸性粒细胞和中性粒细胞趋化活性以及 IL-8 水平。结果：攻击后 5 分钟，与基线相比没有观察到任何变化。攻击后 4 小时，发现中性粒细胞数量和 IL-8 水平有所增加，这取决于 AA 和 ANA 组中过敏原的剂量。在相同的过敏原剂量下，AA 组中性粒细胞和 IL-8 水平的增加分别比 ANA 组高 91 倍和 67 倍。 IL-8 水平与中性粒细胞数量以及 BAL 液中的体外中性粒细胞趋化活性相关。结论：节段性过敏原激发后 4 小时，BAL 液中的中性粒细胞趋化活性增加。我们认为，除了 IgE 介导的肥大细胞脱颗粒之外，气道中的其他局部因素也决定了 IL-8 增加和中性粒细胞流入的程度。
Background: Segmental allergen challenge is a powerful tool to study inflammatory reactions in asthmatic airways. There is little information on the early events at 5 min and 4 h after allergen challenge with respect to the cell influx and the chemokine interleukin-8 (IL-8). Methods: Seven mild to moderate allergic asthmatics (AA group), 5 allergic nonasthmatics (ANA group) and 5 nonallergic controls underwent segmental allergen challenge, with allergen doses based upon skin reactivity. Bronchoalveolar ravage (BAL) samples were obtained before, 5 min and 4 h postchallenge, and were analyzed for cell numbers and differential counts, eosinophil and neutrophil chemotactic activity, and levels of IL-8. Results: At 5 min postchallenge, no changes were observed compared to baseline. At 4 h postchallenge, an increase was found in the number of neutrophils and the levels of IL-8, which was dependent on the dose of allergen in the AA and ANA group. At the same allergen dose, the increases in neutrophils and levels of IL-8 were calculated to be 91 and 67 times higher, respectively, in AA than in ANA. Levels of IL-8 correlated with the number of neutrophils and with the in vitro neutrophil chemotactic activities in BAL fluid. Conclusions: Neutrophil chemotactic activity is increased in BAL fluid at 4 h after segmental allergen challenge. We suggest that apart from IgE-mediated mast cell degranulation, additional local factors in the airways determine the degree of IL-8 increase and neutrophil influx.