Human immunodeficiency virus infection of the developing human nervous system.
Human immunodeficiency virus infection of the developing human nervous system.
复制标题
人类免疫缺陷病毒感染发育中的人类神经系统。
DOI：
10.1016/0042-6822(87)90483-1
复制
发表时间：
1987
期刊：
影响因子：
3.7
通讯作者：
Sarin,PS
中科院分区：
文献类型：
--
作者：
Wigdahl,B;Guyton,RA;Sarin,PS
人类免疫缺陷病毒（HIV）是获得性免疫缺陷综合征（艾滋病）和艾滋病相关复合体的病原，最近被认为是艾滋病相关神经功能障碍发展的一个因素，可能是越来越多的新生儿免疫和神经系统疾病的原因。然而，到目前为止，还没有模型系统可用于研究HIV与体外发育的人类神经系统的相互作用。为了接近与人类胎儿神经系统HIV感染相关的细胞内事件，我们感染了通过酶解流产的人类胎儿背根神经节及其附着的脊髓根和神经获得的细胞。在非神经元形态的细胞亚群中检测到HIVgaggene蛋白产物（p17和p24）的表达，在3天内达到最大值。尽管70%的非神经元细胞在感染后3天呈p17-和p24阳性，但大多数细胞群在急性HIV感染后存活，p17和p24的表达在感染后12天降至检测极限以下。该系统可用于检查神经病理学和神经生物学的急性，持续性，或潜伏HIV感染的发展中的人类神经系统。
Human immunodeficiency virus (HIV), the etiologic agent of acquired immune deficiency syndrome (AIDS) and AIDS-related complex, has recently been implicated as a factor in the development of AIDS-related neurologic dysfunction and may be responsible for an increasing number of neonatal immunologic and neurologic disorders. However, as yet there is no model system available to investigate the interaction of HIV with the developing human nervous systemin vitro. To approximate the intracellular events associated with HIV infection of the human fetus nervous system we infected cells obtained by enzymatic dissociation of aborted human fetus dorsal root ganglia and their attached spinal roots and nerves. The expression of the HIVgaggene protein products (p17 and p24) was detected in a subpopulation of cells with a nonneuronal morphology, reaching a maximum within 3 days. Although 70% of the nonneuronal cells were p17- and p24-positive 3 days after infection, a majority of the cell population survived acute HIV infection, with the expression of p17 and p24 decreasing below the limit of detection by 12 days postinfection. This system may prove useful for examining the neuropathology and neurobiology of acute, persistent, or latent HIV infection of the developing human nervous system.