Fabrication of an Oriented Lectin Microarray
Fabrication of an Oriented Lectin Microarray
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DOI：
10.1002/cbic.201000106
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发表时间：
2010-06-14
期刊：
影响因子：
3.2
通讯作者：
Mahal, Lara K.
中科院分区：
文献类型：
--
作者：
Propheter, Daniel C.;Hsu, Ku-Lung;Mahal, Lara K.
标记或不标记：一个定向凝集素微阵列利用重组凝集素与标准原核表达标记谷胱甘肽s -转移酶已被报道。定向凝集素微阵列提高了重组凝集素的总体灵敏度和位点特异性定向检测的限制。凝集素微阵列技术的不断改进使人们能够更深入地了解聚糖的生物学功能。(图)
To tag or not to tag: An oriented lectin microarray utilizing recombinant lectins with the standard prokaryotic expression tag glutathione S-transferase has been reported. The oriented lectin microarray improves the overall sensitivity and limits of detection by site-specific orientation of recombinant lectins. This continual improvement of the lectin microarray technology enables a deeper understanding of the biological function of glycans.(Figure Presented)