Identification and comparative expression analysis of a second wt1 gene in zebrafish
Identification and comparative expression analysis of a second wt1 gene in zebrafish
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DOI：
10.1002/dvdy.20645
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发表时间：
2006-02-01
影响因子：
2.5
通讯作者：
Englert, C
中科院分区：
文献类型：
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作者：
Bollig, F;Mehringer, R;Englert, C
威尔姆斯氏肿瘤抑制基因wt1编码锌指转录因子，该因子在哺乳动物泌尿生殖系统的发育中发挥重要作用。人类 WT1 突变会导致肾脏和性腺发育异常，并导致肾母细胞瘤（一种小儿肾癌）。小鼠体内两个 wt1 等位基因的失活会导致多种器官缺陷，其中包括肾脏、脾脏和性腺发育不全。在斑马鱼中，wt1 的直系同源物已被描述，它在前肾区表达，后来仅限于足细胞。在这里，我们报告了斑马鱼中存在第二个 wt1 基因，我们将其命名为 wt1b（我们将初始基因命名为 wt1a）。两种 Wt1 蛋白锌指区域之间的总体序列同一性分别为 70% 和 92%。与 wt1a 相比，wt1b 从发育的最早阶段开始表达，尽管水平较低。 wt1a 和 wt1b 均在中间中胚层中表达，其中 wt1b 仅限于位于 wt1a 表达结构域尾端的较小区域。在成年鱼类中，这两种基因在性腺、肾脏、心脏、脾脏和肌肉中都有高表达。
The Wilms' tumor suppressor gene wt1 encodes a zinc-finger transcription factor that plays an important role in the development of the mammalian genitourinary system. Mutations in WT1 in humans lead to anomalies of kidney and gonad development and cause Wilms' tumor, a pediatric kidney cancer. The inactivation of both wt1 alleles in mice gives rise to multiple organ defects, among them agenesis of kidney, spleen, and gonads. In zebrafish, an ortholog of wt1 has been described that is expressed in the pronephric field and is later restricted to the podocytes. Here, we report the existence of a second wt1 gene in zebrafish, which we have named wt1b (we named the initial gene wt1a). The overall sequence identity of the two Wt1 proteins is 70% and 92% between the zinc-finger regions, respectively. In contrast to wt1a, wt1b is expressed from the earliest stages of development onward, albeit at low levels. Both wt1a and wt1b are expressed in the intermediate mesoderm, with wt1b being restricted to a smaller area lying at the caudal end of the wt1a expression domain. In adult fish, high expression levels for both genes can be found in gonads, kidney, heart, spleen, and muscle.