HIV-1 gp120-induced apoptosis in the rat neocortex involves enhanced expression of cyclo-oxygenase type 2 (COX-2)
HIV-1 gp120-induced apoptosis in the rat neocortex involves enhanced expression of cyclo-oxygenase type 2 (COX-2)
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DOI：
10.1006/bbrc.1998.8321
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发表时间：
1998-03-27
影响因子：
3.1
通讯作者：
Finazzi-Agrò, A
中科院分区：
文献类型：
--
作者：
Bagetta, G;Corasaniti, MT;Finazzi-Agrò, A
采用免疫组织化学方法研究了亚慢性脑室注射人免疫缺陷病毒1型（HIV-1）重组蛋白gp120 （100 ng，每天给药，连续7天）对成年大鼠脑内环加氧酶2 （COX-2）表达的影响。与对照组相比，牛血清白蛋白（100 ng，静脉注射7天）处理的动物（n=6），连续7天每天注射病毒蛋白增加了大鼠大脑皮层中COX-2免疫反应细胞的数量（每组n=6），这伴随着全脑组织匀浆中PGE(2)含量比对照组增加50% （n=6）。在另一系列实验中，用吲哚美辛（6.0 mg/kg，注射gp120前1小时）预处理大鼠（n=6），抑制COX活性，阻止gp120在大鼠新皮层产生的凋亡，这表明COX-2表达的增强可能参与了HIV-1外壳蛋白产生的凋亡机制。(C) 1998学术出版社。
The effect of subchronic intracerebroventricular (i.c.v.) injection of the human immunodeficiency virus type 1 (HIV-1) recombinant protein gp120 (100 ng, given daily for up to 7 consecutive days) on cyclo-oxygenase type 2 (COX-2) expression was studied by immunohistochemistry in the brain of adult rats. In comparison to control, bovine serum albumin (100 ng, given i.c.v. for up to 7 days) treated animals (n=6), a single daily injection of the viral protein for 7 consecutive days enhanced the number of COX-2 immunoreactive cells in the brain cortex of rats (n=6 per group) and this was accompanied by a 50% increase over control PGE(2) content in whole brain tissue homogenates (n=6). In another series of experiments, pretreatment of rats (n=6) with indomethacin (6.0 mg/kg given i.p. 1 h before gp120 injection), an inhibitor COX activity, prevented apoptotic death typically produced by gp120 in the neocortex of rat suggesting that enhancement of COX-2 expression may be involved in the mechanisms of apoptosis yielded by the HIV-1 coat protein. (C) 1998 Academic Press.