Behavioral Choice between Conflicting Alternatives Is Regulated by a Receptor Guanylyl Cyclase, GCY-28, and a Receptor Tyrosine Kinase, SCD-2, in AIA Interneurons of Caenorhabditis elegans
Behavioral Choice between Conflicting Alternatives Is Regulated by a Receptor Guanylyl Cyclase, GCY-28, and a Receptor Tyrosine Kinase, SCD-2, in AIA Interneurons of Caenorhabditis elegans
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DOI：
10.1523/jneurosci.4691-10.2011
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发表时间：
2011-02-23
影响因子：
5.3
通讯作者：
Ishihara, Takeshi
中科院分区：
文献类型：
--
作者：
Shinkai, Yoichi;Yamamoto, Yuta;Ishihara, Takeshi
动物面对相互冲突的感觉线索，通过整合感觉线索，在相互竞争的替代品之间做出行为选择。在这里，我们进行了遗传筛选，以确定两个相互冲突的线索，有吸引力的气味双乙酰和厌恶刺激铜+的感觉整合的重要基因，并发现，膜结合鸟苷酸环化酶GCY-28和受体酪氨酸激酶SCD-2调节这些替代品之间的行为选择秀丽隐杆线虫。gcy-28突变体和scd-2突变体在对不同线索的行为选择上表现出异常的偏向，尽管它们对每个线索的反应与野生型动物相似。编码环核苷酸门控离子通道cng-1的基因突变体也表现出感觉整合的缺陷。分子遗传学分析表明，GCY-28和SCD-2调节AIA中间神经元的感觉整合，其中相互冲突的感觉线索可能会收敛。AIA中间神经元的基因消融或超极化在感觉整合中显示出与gcy-28或scd-2突变体几乎相同的表型，尽管这并不影响对每个单独提示的感觉反应。在gcy-28或scd-2突变体中，AIA中间神经元的激活足以恢复正常的感觉整合。这些结果表明，AIA中间神经元的活动调节的行为选择之间的选择。我们提出GCY-28和SCD-2通过调节AIA中间神经元的活性来调节感觉整合。
Animals facing conflicting sensory cues make a behavioral choice between competing alternatives through integration of the sensory cues. Here, we performed a genetic screen to identify genes important for the sensory integration of two conflicting cues, the attractive odorant diacetyl and the aversive stimulus Cu2+, and found that the membrane-bound guanylyl cyclase GCY-28 and the receptor tyrosine kinase SCD-2 regulate the behavioral choice between these alternatives in Caenorhabditis elegans. The gcy-28 mutants and scd-2 mutants show an abnormal bias in the behavioral choice between the cues, although their responses to each individual cue are similar to those in wild-type animals. Mutants in a gene encoding a cyclic nucleotide gated ion channel, cng-1, also exhibit the defect in sensory integration. Molecular genetic analyses suggested that GCY-28 and SCD-2 regulate sensory integration in AIA interneurons, where the conflicting sensory cues may converge. Genetic ablation or hyperpolarization of AIA interneurons showed nearly the same phenotype as gcy-28 or scd-2 mutants in the sensory integration, although this did not affect the sensory response to each individual cue. In gcy-28 or scd-2 mutants, activation of AIA interneurons is sufficient to restore normal sensory integration. These results suggest that the activity of AIA interneurons regulates the behavioral choice between the alternatives. We propose that GCY-28 and SCD-2 regulate sensory integration by modulating the activity of AIA interneurons.