A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1.
A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1.
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DOI：
10.4049/jimmunol.137.4.1270
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发表时间：
1986-08
影响因子：
4.4
通讯作者：
R. Rothlein;Michael Loran Dustin;S. D. Marlin;T. Springer
中科院分区：
文献类型：
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作者：
R. Rothlein;Michael Loran Dustin;S. D. Marlin;T. Springer
佛波酯刺激的淋巴细胞的同型黏附需要LFA-1和mg+2，不涉及LFA-1分子与相邻细胞之间的类样相互作用。后者的发现表明，与LFA-1不同的第二个分子也参与了LFA-1依赖的黏附。这种分子的鉴定就是本次调查的对象。用LFA-1缺陷的EBV转化的淋巴母细胞免疫后，获得了一株能抑制佛波酯刺激的LFA-1+EBV株聚集的单抗。该单抗定义了一种新的细胞表面分子，命名为细胞间黏附分子1(ICAM-1)。ICAM-1在细胞分布和结构上都不同于LFA-1。在SDS-PAGE中，从JY细胞中分离到的ICAM-1是一条Mr=90,000的单链。单抗抑制实验表明，ICAM-1参与佛波酯刺激的B淋巴细胞、髓系细胞和T淋巴细胞的黏附反应。但LFA-1可抑制一种T淋巴细胞株(SKW-3)的聚集，而ICAM-1单抗不能抑制其聚集。有人认为ICAM-1可能是许多(但不是全部)依赖LFA-1的黏附反应的配体。
Homotypic adhesion by phorbol ester-stimulated lymphocytes requires LFA-1 and Mg+2 and does not involve like-like interactions between LFA-1 molecules on adjacent cells. The latter finding suggested that a second molecule, distinct from LFA-1, also participates in LFA-1-dependent adhesion. The identification of such a molecule was the object of this investigation. After immunization with LFA-1-deficient EBV-transformed lymphoblastoid cells, a MAb was obtained that inhibits phorbol ester-stimulated aggregation of LFA-1+ EBV lines. This MAb defines a novel cell surface molecule, which is designated intercellular adhesion molecule 1 (ICAM-1). ICAM-1 is distinct from LFA-1 in both cell distribution and structure. In SDS-PAGE, ICAM-1 isolated from JY cells is a single chain of Mr = 90,000. As shown by MAb inhibition, ICAM-1 participates in phorbol ester-stimulated adhesion reactions of B lymphocyte and myeloid cell lines and T lymphocyte blasts. However, aggregation of one T lymphocyte cell line (SKW-3) was inhibited by LFA-1 but not ICAM-1 MAb. It is proposed that ICAM-1 may be a ligand in many, but not all, LFA-1-dependent adhesion reactions.