Modeling Neurodegenerative Diseases in Zebrafish Embryos
Modeling Neurodegenerative Diseases in Zebrafish Embryos
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DOI：
10.1007/978-1-61779-328-8_11
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发表时间：
2011-01-01
期刊：
影响因子：
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通讯作者：
Robberecht, Wim
中科院分区：
文献类型：
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作者：
Laird, Angela S.;Robberecht, Wim
尽管斑马鱼（Danio rerio）已被广泛用于神经发育研究多年，但使用这种硬骨鱼来研究神经系统疾病只是最近才发展起来的。作为一种脊椎动物，这种动物比其他小动物（如苍蝇或蠕虫）更有利于研究人类疾病。遗传性和非遗传性疾病都可以在成人机体和胚胎中建立模型。通过对胚胎进行基因操作来产生稳定和瞬时表达的转基因鱼，以及敲低基因来研究其功能的丧失，都是很容易实现的。由于后代数量多，筛选研究也可以很容易地进行。在这里，我们描述了一些对斑马鱼胚胎中神经退行性疾病建模有用的方案，特别强调了研究运动神经元表型的模型。
Although the zebrafish (Danio rerio) has been used extensively for many years in neurodevelopmental studies, use of this teleost to study neurological diseases has evolved only recently. Being a vertebrate, this animal offers advantages for the study of human disease over other small animals, such as the fly or worm. Genetic, as well as nongenetic, disorders can be modeled in both the adult organism and the embryo. Genetic manipulation of the embryo to generate stable and transiently expressing transgenic fish, and to knockdown genes to study loss of their function, can be easily achieved. Because of large offspring numbers screening studies can also be readily performed. Here, we describe some of the protocols useful for modeling neurodegenerative disease in zebrafish embryos, with particular emphasis on models to study motor neuron phenotypes.