Sex differences in expression and subcellular localization of heart rhythm determinant proteins.
Sex differences in expression and subcellular localization of heart rhythm determinant proteins.
复制标题
心律决定蛋白的表达和亚细胞定位的性别差异。
DOI：
10.1016/j.bbrc.2011.02.006
复制
发表时间：
2011
影响因子：
3.1
通讯作者：
Iacobas,DA
中科院分区：
文献类型：
--
作者：
Thomas,NM;Jasmin,JF;Lisanti,MP;Iacobas,DA
为了评估心脏中蛋白质表达的性别差异，我们对心脏节律决定因子（HRD）蛋白的一个子集进行了Western印迹研究。我们研究了各种类型的机械和电气连接的关键组成部分，包括，连接蛋白43，斑嗜蛋白-2，N-钙粘蛋白和斑珠蛋白，锚蛋白-2和肌动蛋白。我们描述了心脏蛋白表达和膜定位的性别差异的新发现。对于大多数蛋白质检查，性别差异显着更明显的膜室比整体表达。这些研究扩展了我们之前在微阵列研究中的发现，证明基因表达的性别差异可能赋予男性和女性心肌不同的功能特性。
To evaluate sex differences in protein expression in the heart, we performed Western blot studies on a subset of Heart Rhythm Determinant (HRD) proteins. We examined key components of a variety of types of mechanical and electrical junctions including, connexin43, plakophilin-2, N-cadherin and plakoglobin, ankyrin-2 and actin. We describe novel findings in sex differences in cardiac protein expression and membrane localization. For most proteins examined, sex differences were significantly more pronounced in the membrane compartment than in overall expression. These studies extend our previous findings in microarray studies to demonstrate that sex differences in gene expression are likely to confer distinct functional properties on male and female myocardium.