Orchestrating redox signaling networks through regulatory cysteine switches.
Orchestrating redox signaling networks through regulatory cysteine switches.
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DOI：
10.1021/cb900258z
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发表时间：
2010-01-15
影响因子：
4
通讯作者：
Carroll KS
中科院分区：
文献类型：
--
作者：
Paulsen CE;Carroll KS
过氧化氢（H2O2）作为第二信使，可通过化学选择性氧化信号蛋白中的半胱氨酸残基介导细胞内信号转导。本文综述了信号介导的H2O2产生的机制，并强调了在体外和细胞中检测活性氧（ROS）和半胱氨酸氧化的方法的最新进展。从最近的文献中选择的例子来说明H2O2可以调节蛋白质功能的不同机制。检测和定量离散半胱氨酸氧化形式的方法的持续发展应进一步促进我们对蛋白质功能的氧化还原调节的机械理解，并可能导致新的治疗策略的发展。
Hydrogen peroxide (H2O2) acts as a second messenger that can mediate intracellular signal transduction via chemoselective oxidation of cysteine residues in signaling proteins. This Review presents current mechanistic insights into signal-mediated H2O2 production and highlights recent advances in methods to detect reactive oxygen species (ROS) and cysteine oxidation both in vitro and in cells. Selected examples from the recent literature are used to illustrate the diverse mechanisms by which H2O2 can regulate protein function. The continued development of methods to detect and quantify discrete cysteine oxoforms should further our mechanistic understanding of redox regulation of protein function and may lead to the development of new therapeutic strategies.