Genetic relationships and clonal identity in a collection of commercially relevant poplar cultivars assessed by AFLP and SSR
Genetic relationships and clonal identity in a collection of commercially relevant poplar cultivars assessed by AFLP and SSR
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DOI：
10.1007/s11295-004-0002-9
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发表时间：
2005-05-01
影响因子：
2.4
通讯作者：
Castiglione, Stefano
中科院分区：
文献类型：
--
作者：
Fossati, Tiziana;Zapelli, Ilaria;Castiglione, Stefano
利用扩增片段长度多态性(AFLP)和简单序列重复序列(SSR)技术，对在意大利、中国和南欧其他国家广泛种植的66个杨树商业无性系进行了指纹分析，这些无性系属于不同的杨树物种和杂种。三个AFLP引物组合和六个SSR明确地对所分析的杨树样本进行了基因分型，但六个、四个和两个个体的三组除外，事实证明，即使它们符合目前注册时应用的区分性、一致性和稳定性(DUS)测试标准，也是无法区分的。两种分子标记技术都检测到了较高的变异水平，共扩增出201条AFLP带，其中96%为多态，在一个座位上可检测到多达15个SSR等位基因，平均每个座位有9.3个等位基因。在Rx加拿大病例中，所有SSR基因座的任意两个基因座碰巧匹配的概率小于7.5×10(-9)。基于AFLP的树状图和主坐标分析(PCOORDA)根据无性系的分类对它们进行了聚类，并建立了它们的遗传关系。正确识别杨树品种对于确保克隆人的真实身份和声明身份之间的有效对应、避免商业欺诈和制定育种计划至关重要。分子标记可能在满足所有这些需求方面发挥重要作用。
A collection of 66 poplar commercial clones widely cultivated in Italy, China and in other countries of southern Europe and belonging to various poplar species and hybrids, have been fingerprinted using both amplified fragment length polymorphism (AFLP) and simple sequence repeats (SSR) techniques. Three AFLP primer combinations and six SSRs unambiguously genotyped the analysed poplar collection, with the exception of three groups of six, four and two individuals, which turned out to be indistinguishable even if they met the standards currently applied for distinctness, uniformity and stability (DUS) testing when registered. High levels of variation were detected with both molecular techniques; a total of 201 AFLP bands were amplified of which 96% turned out to be polymorphic and up to 15 SSR alleles were identified at a single locus, with a mean of 9.3 alleles per locus in the case of Populus x canadensis. The probability of matching fortuitously any two genotypes at all the SSR loci in the case of R x canadensis was less then 7.5x10(-9). The AFLP-derived dendrogram and principal coordinate analysis (PCOORDA) clustered the clones with respect to their taxonomic classification, and allowed their genetic interrelationships to be established. Correct identification of poplar varieties is essential for ensuring the effective correspondence between the real and the declared identity of a clone, to avoid commercial frauds, and to establish breeding programmes. Molecular markers may play a major role to satisfy all these needs.