N6-methyl adenosine in siRNA evades immune response without reducing RNAi activity
N6-methyl adenosine in siRNA evades immune response without reducing RNAi activity
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DOI：
10.1080/15257770.2019.1641205
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发表时间：
2019-07-11
影响因子：
1.3
通讯作者：
Abe, Hiroshi
中科院分区：
文献类型：
--
作者：
Imaeda, Akihiro;Tomoike, Fumiaki;Abe, Hiroshi
siRNA是抑制特定基因表达的有效方法，最近已被用于分子生物学以及医学。然而，dsRNA的引入刺激免疫应答作为副作用。在本研究中，我们利用N-6-甲基腺苷，一种天然修饰的核苷，代替siRNA中的腺苷。当siRNA的过客链或引导链中的腺苷被N-6-甲基腺苷完全取代时，针对siRNA的免疫应答被回避而不降低RNAi活性。这些知识将促进siRNA在医学上的应用，并加深我们对非自身和自身dsRNA的细胞识别的理解。
siRNA is a powerful method to suppress specific gene expression and has recently been utilized for molecular biology as well as medicine. However, introduction of dsRNA stimulates immune-responses as side-effects. In the present study, we utilized N-6-methyl adenosine, one of the natural modified nucleosides, instead of adenosine in siRNA. When adenosine in the passenger or guide strand of siRNA was completely replaced with N-6-methyl adenosine, the immune response against siRNA was evaded without any reduction in RNAi activity. This knowledge will promote the medical application of siRNA and enhance our understanding on cellular discrimination of non-self and self dsRNA.