Enzyme Degradable Polymersomes from Hyaluronic Acid-block-poly(ε-caprolactone) Copolymers for the Detection of Enzymes of Pathogenic Bacteria
Enzyme Degradable Polymersomes from Hyaluronic Acid-block-poly(ε-caprolactone) Copolymers for the Detection of Enzymes of Pathogenic Bacteria
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DOI：
10.1021/bm501729h
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发表时间：
2015-03-01
影响因子：
6.2
通讯作者：
Schoenherr, Holger
中科院分区：
文献类型：
--
作者：
Haas, Simon;Hain, Nicole;Schoenherr, Holger
我们介绍了一种新的透明质酸酶响应的两亲性嵌段共聚物系统，基于透明质酸（HYA）和聚己内酯（PCL），可以组装成聚合物囊泡的反溶剂转移方法。通过利用包封的染料分子的触发释放，这些HYA-嵌段-PCL聚合物囊泡本身作为用于检测透明质酸酶的存在的自主感测系统，其中透明质酸酶由致病性细菌金黄色葡萄球菌产生。酶响应性HYA-嵌段-PCL嵌段共聚物的合成通过ω-叠氮化物封端的PCL和ω-炔官能化的HYA的铜催化的Huisgen 1，3-偶极环加成进行。HYA-块-PCL组件的结构和它们的酶触发的降解和伴随的货物释放进行了研究，通过动态光散射，荧光光谱，共聚焦激光扫描显微镜，扫描和透射电子显微镜，和原子力显微镜。如图所示，在形成过程中，多种报道染料分子以及抗微生物剂可以包封到囊泡中，并在加入透明质酸酶后释放。
We introduce a new hyaluronidase-responsive amphiphilic block copolymer system, based on hyaluronic acid (HYA) and polycaprolactone (PCL), that can be assembled into polymersomes by an inversed solvent shift method. By exploiting the triggered release of encapsulated dye molecules, these HYA-block-PCL polymersomes lend themselves as an autonomous sensing system for the detection of the presence of hyaluronidase, which is produced among others by the pathogenic bacterium Staphylococcus aureus. The synthesis of the enzyme-responsive HYA-block-PCL block copolymers was carried out by copper-catalyzed Huisgen 1,3-dipolar cycloaddition of omega-azide-terminated PCL and omega-alkyne-functionalized HYA. The structure of the HYA-block-PCL assemblies and their enzyme-triggered degradation and concomitant cargo release were investigated by dynamic light scattering, fluorescence spectroscopy, confocal laser-scanning microscopy, scanning and transmission electron, and atomic force microscopy. As shown, a wide range of reporter dye molecules as well as antimicrobials can be encapsulated into the vesicles during formation and are released upon the addition of hyaluronidase.