STAIRS:: a new genetic resource for functional genomic studies of Arabidopsis
STAIRS:: a new genetic resource for functional genomic studies of Arabidopsis
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DOI：
10.1046/j.1365-313x.2002.01353.x
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发表时间：
2002-08-01
期刊：
影响因子：
7.2
通讯作者：
Kearsey, MJ
中科院分区：
文献类型：
--
作者：
Koumproglou, R;Wilkes, TM;Kearsey, MJ
植物和动物的许多重要的生物学和经济学性状是数量/多因子的，由多个数量性状基因座（QTL）控制。在分离群体中，利用分子标记的常规方法很难准确定位QTL，特别是对于遗传力低的性状或效应小的QTL。为了解决这个问题，需要大量（通常是不现实的大量）人口。在本文中，我们提出了一种替代方法，使用专门开发的资源线，促进QTL定位首先到一个特定的染色体，然后连续较小的区域内的染色体（小于或等于0.5厘米）通过简单的比较几行。该资源由“逐步对齐的近交重组菌株”（STAIRS）加上单一的全染色体置换菌株（CSS）组成。我们解释了分析能力的STAIRS和说明其建设和使用拟南芥，虽然原则可以适用于许多生物。我们能够定位开花QTL在3号染色体的顶部已知含有几个潜在的候选基因。
Many biologically and economically important traits in plants and animals are quantitative/multifactorial, being controlled by several quantitative trait loci (QTL). QTL are difficult to locate accurately by conventional methods using molecular markers in segregating populations, particularly for traits of low heritability or for QTL with small effects. In order to resolve this, large (often unrealistically large) populations are required. In this paper we present an alternative approach using a specially developed resource of lines that facilitate QTL location first to a particular chromosome, then to successively smaller regions within a chromosome (less than or equal to 0.5 cM) by means of simple comparisons among a few lines. This resource consists of 'Stepped Aligned Inbred Recombinant Strains' (STAIRS) plus single whole Chromosome Substitution Strains (CSSs). We explain the analytical power of STAIRS and illustrate their construction and use with Arabidopsis thaliana, although the principles could be applied to many organisms. We were able to locate flowering QTL at the top of chromosome 3 known to contain several potential candidate genes.