Citric Acid Passivation of Titanium Dental Implants for Minimizing Bacterial Colonization Impact
Citric Acid Passivation of Titanium Dental Implants for Minimizing Bacterial Colonization Impact
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DOI：
10.3390/coatings11020214
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发表时间：
2021-02-01
期刊：
影响因子：
3.4
通讯作者：
Gil, Javier
中科院分区：
文献类型：
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作者：
Punset, Miquel;Vilarrasa, Javi;Gil, Javier
牙种植体表面的表面形貌和物理化学性质通常在改善骨整合和减少细菌在种植体表面的定植方面起着关键作用。本研究的目的是比较两种不同酸钝化表面处理的钛C.P.的化学和细菌学行为。3级，用于牙种植体制造。为了确定不同的粗糙度参数，如平均粗糙度(Sa)、间距参数(Sm)和表面指数面积的混合参数(Sia)，利用白光干涉法(WLI)对表面粗糙度进行了评估。为了确定不同组样品的表面润湿性，对接触角(CA)和表面自由能(SFE)进行了评估。用电感耦合等离子体质谱(ICP-MS)分析了不同样品在37℃的Hank‘s溶液介质中不同浸泡时间的钛离子释放情况。用血链球菌(CECT480，西班牙)作为口腔生物膜形成主要定殖物的细菌模型进行细菌活性黏附试验。孵育4h后，用活/死染色方法检测细菌在钛表面的附着和形态，并用扫描电子显微镜(SEM)进一步分析。酸钝化表面处理使所有评价参数(Sa、Sm、SAI)的粗糙度均有统计学意义的增加(p<；0.05)。柠檬酸处理降低了静态接触角(CA)，增加了表面自由能(SFE)，具有较高的极化和氧化特性。通过柠檬酸钝化获得的这些物理化学表面特征导致了杀菌行为，这在细菌研究中得到了证实。
Surface topography and physical-chemical properties usually play a key-role in both osseointegration improvement and bacterial colonization reduction over the surface of dental implants. The aim of this study is to compare the chemical and bacteriological behavior of two different acid passivation surface treatments on titanium c.p. grade 3 used for dental implant manufacturing. Surface roughness was evaluated using White Light Interferometry (WLI) in order to determine different roughness parameters such as average roughness (Sa), the spacing parameter (Sm) and the hybrid parameter of surface index area (SIA). Contact angle (CA) and surface free energy (SFE) were evaluated in order to establish the surface wettability of the different groups of samples. Titanium ion-release from the different samples was also been analyzed in Hank's solution medium at 37 degrees C by using Inductively Coupled Plasma Mass Spectrometry (ICP-MS) at different immersion times. Bacterial viability adhesion assays were done using S. sanguinis (CECT 480, Spain) as a bacterial strain model of primary colonizer in oral biofilm formation. The bacteria attachment and morphology on Ti surfaces were determined using a live/dead staining method after 4 h of incubation and further analyzed by scanning electron microscope (SEM). Acid passivation surface treatments produced a statistically-significant (p < 0.05) roughness increase in all the evaluated parameters (Sa, Sm, SAI). The treatment with citric acid decreased the static contact angle (CA) and caused an increase in surface free energy (SFE) with a high polarization and oxidizing character. These physical-chemical surface characteristics obtained by means of citric acid passivation caused the bactericidal behavior as it has been proved in bacterial studies.