Proteomic Analysis of Silk Viability in Maize Inbred Lines and Their Corresponding Hybrids.
Proteomic Analysis of Silk Viability in Maize Inbred Lines and Their Corresponding Hybrids.
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玉米自交系及其相应杂交种丝活力的蛋白质组学分析
DOI：
10.1371/journal.pone.0144050
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Fu Z
中科院分区：
文献类型：
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作者：
Ma Z;Qin Y;Wang Y;Zhao X;Zhang F;Tang J;Fu Z
长时期的生丝活力是玉米（Zea mays L.）良好结实率的关键，特别是对于自交系和开花吐丝间隔较长的杂交种。为探讨蚕丝活力及其杂种优势的分子机制，采用蛋白质组学方法对蚕丝活力特征不同的3个自交系（Xun928、Lx9801和宗3）及其2个杂交种（Xun928×Zong3和Lx9801×Zong3）在不同发育阶段进行了分析。通过质谱法鉴定了差异积累蛋白，并将其分为代谢、蛋白质生物合成和折叠、信号转导和激素稳态、应激和防御反应以及细胞过程。蚕丝中参与营养（蛋氨酸）和能量（ATP）供应的蛋白质支持花粉管的生长，对蚕丝的生存和杂种优势具有重要意义。单个位点的加性和显性效应以及代谢途径中两个或多个位点的复杂上位相互作用是蚕丝中亲本杂种优势的主要原因。此外，参与花青素代谢的蛋白质间接负调控局部激素积累，对蚕丝生存力的中亲本杂种优势也很重要。这些结果也可能暗示了杂种优势的发育依赖性，因为许多差异积累的蛋白质在特定发育阶段对丝活力的杂种优势有不同的贡献。
A long period of silk viability is critical for a good seed setting rate in maize (Zea mays L.), especially for inbred lines and hybrids with a long interval between anthesis and silking. To explore the molecular mechanism of silk viability and its heterosis, three inbred lines with different silk viability characteristics (Xun928, Lx9801, and Zong3) and their two hybrids (Xun928×Zong3 and Lx9801×Zong3) were analyzed at different developmental stages by a proteomic method. The differentially accumulated proteins were identified by mass spectrometry and classified into metabolism, protein biosynthesis and folding, signal transduction and hormone homeostasis, stress and defense responses, and cellular processes. Proteins involved in nutrient (methionine) and energy (ATP) supply, which support the pollen tube growth in the silk, were important for silk viability and its heterosis. The additive and dominant effects at a single locus, as well as complex epistatic interactions at two or more loci in metabolic pathways, were the primary contributors for mid-parent heterosis of silk viability. Additionally, the proteins involved in the metabolism of anthocyanins, which indirectly negatively regulate local hormone accumulation, were also important for the mid-parent heterosis of silk viability. These results also might imply the developmental dependence of heterosis, because many of the differentially accumulated proteins made distinct contributions to the heterosis of silk viability at specific developmental stages.