The reaction pathway of membrane-bound rat liver mitochondrial monoamine oxidase.
The reaction pathway of membrane-bound rat liver mitochondrial monoamine oxidase.
复制标题
膜结合型大鼠肝线粒体单胺氧化酶的反应途径。
作者：
M. Houslay;K. Tipton
1. A preparation of a partly purified mitochondrial outer-membrane fraction suitable for kinetic investigations of monoamine oxidase is described. 2. An apparatus suitable for varying the O(2) concentration in a spectrophotometer cuvette is described. 3. The reaction catalysed by the membrane-bound enzyme is shown to proceed by a double-displacement (Ping Pong) mechanism, and a formal mechanism is proposed. 4. KCN, NaN(3), benzyl cyanide and 4-cyanophenol are shown to be reversible inhibitors of the enzyme. 5. The non-linear reciprocal plot obtained with impure preparations of benzylamine, which is typical of high substrate inhibition, is shown to be due to aldehyde contamination of the substrate.
