[Establishment of a cisplatin-resistant new human endometrial adenocarcinoma cell line, Sawano cells].
[Establishment of a cisplatin-resistant new human endometrial adenocarcinoma cell line, Sawano cells].
复制标题
人子宫内膜腺癌细胞顺铂耐药新细胞系泽野细胞的建立
作者：
T. Satoh;M. Nishida;Y. Miyazaki;M. Sugita;Y. Arai;A. Oki;K. Kono;H. Tsunoda;K. Kasahara;T. Kubo
新的人类子宫内膜腺癌细胞系Sawano细胞是从一名61岁女性的子宫内膜腺癌中建立的，并在体外维持了2年零10个月以上。发现这些细胞以镶嵌方式形成单层并且倾向于堆积。第10代的群体倍增时间为43.2小时。细胞的染色体众数落在二倍体范围内。无胸腺小鼠中诱导的肿瘤的组织学显示，它是一种中度分化的腺癌，与原始人类肿瘤非常相似。尽管雌激素和孕激素受体在原始肿瘤中得到了阳性证实，但在体外培养细胞和裸鼠诱导的肿瘤中并未得到证实。接触120小时后，细胞具有内在的顺铂抗性（50%抑制浓度：6.63微克/ml）。我们相信该细胞系有助于我们研究 CDDP 耐药性的生物学机制。
A new human endometrial adenocarcinoma cell line, Sawano cells, was established from an endometrial adenocarcinoma from a 61-year-old woman and has been maintained in vitro for more than 2 years and 10 months. The cells were found to form a monolayer in a mosaic fashion and to tend to pile up. Population doubling time was 43.2 hours at the 10th generation. The modal chromosomal number of the cell fell in a diploid range. Histology of the tumor induced in athymic mice showed it to be a moderately differentiated adenocarcinoma which closely resembled the original human tumor. Estrogen and progesterone receptors were not demonstrated in the in vitro culture cells and in the tumors induced in nude mice, though they were positively demonstrated in the original tumor. The cells had intrinsic cisplatin-resistance (50% inhibition concentration:6.63 micrograms/ml) at 120 hours of contact. We believe this cell line with help us to investigate the biological mechanisms of CDDP resistance.