Bulked segregant analysis for relative water content to detect quantitative trait loci in wheat under drought stress
Bulked segregant analysis for relative water content to detect quantitative trait loci in wheat under drought stress
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DOI：
10.4238/2012.november.12.5
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发表时间：
2012-01-01
影响因子：
0.4
通讯作者：
Naghavi, M. R.
中科院分区：
文献类型：
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作者：
Rad, M. R. Naroui;Kadir, M. Abdul;Naghavi, M. R.
本研究旨在评估赋予小麦耐旱性的数量性状基因座（QTL）的遗传效应。分析了来自耐旱 S-78-11 和干旱敏感 Tajan 品种杂交的 120 个 F-2 个体在干旱胁迫条件下的分离情况。干旱胁迫条件下的相对含水量表现出持续变化，表明基因对性状的影响较小。进行单标记分析（SMA）来检测与耐旱性的主要QTL关联。 SMA结果显示，5D染色体上的简单序列重复标记GWM182和GWM292以及5A染色体上的GWM410与耐旱性显着相关，分别占总变异的30%、22%和21%。这3个基因位点，尤其是GWM182，可用于小麦耐旱育种的标记辅助选择方法。
This study was carried out to evaluate the genetic effect of quantitative trait loci (QTLs) conferring drought tolerance in wheat. A population of 120 F-2 individuals from the cross between the drought-tolerant S-78-11 and drought-sensitive Tajan cultivars were analyzed for their segregation under drought stress conditions. The relative water content under drought stress conditions exhibited continuous variation, indicating the minor gene effects on the trait. Single-marker analysis (SMA) was carried out to detect the main QTL association with drought tolerance. The SMA results revealed that the simple sequence repeat markers GWM182 and GWM292 on chromosome 5D and GWM410 on chromosome 5A exhibited significant association with drought tolerance, accounting for 30, 22, and 21% of the total variation, respectively. The 3 genetic loci, especially GWM182, can be used in marker-assisted selection methods in drought tolerance breeding in wheat.