DEFINITION OF 2 NEW GROUPS OF ATYPICAL ROTAVIRUSES
DEFINITION OF 2 NEW GROUPS OF ATYPICAL ROTAVIRUSES
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DOI：
10.1099/0022-1317-67-1-131
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发表时间：
1986-01-01
影响因子：
3.8
通讯作者：
MCCRAE, MA
中科院分区：
文献类型：
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作者：
PEDLEY, S;BRIDGER, JC;MCCRAE, MA
对来自鸡(D/132)和猪(E/DC-9)的两个新的非典型轮状病毒分离株进行了抗原性和核酸比较分析。间接免疫荧光显示，每种病毒携带不同的组抗原，这些抗原也与以前描述的轮状病毒组不同。通过基因组图谱分析，每种病毒都有明显不同于其他轮状病毒组的基因组RNA模式。两种病毒相应基因组片段的比较末端指纹图谱显示，它们之间存在较大差异，表明它们的所有基因组RNA在序列上彼此之间以及与先前定义的三个轮状病毒组的序列都存在显著差异。根据这些结果，建议将轮状病毒群的数量从3个扩大到5个，D/132和E/DC-9分离株分别是D和E群的模式成员。
Comparative antigenic and nucleic acid analyses were carried out on two new atypical rotavirus isolates coming respectively from chickens (D/132) and pigs (E/DC-9). Indirect immunofluorescence showed that each virus carried different group antigens which were also distinct from those of previously described rotavirus groups. By genome profile analysis each virus had a pattern of genomic RNAs clearly distinct from those of the other rotavirus groups. Comparative terminal fingerprinting of corresponding genome segments from the two viruses showed large differences between them, indicating that all of their genomic RNAs had significant differences in sequence both from each other and from the three previously defined rotavirus groups. On the basis of these results, extension of the number of rotavirus groups from three to five is proposed, with isolates D/132 and E/DC-9 being the type members of groups D and E respectively.