A brassinosteroid transcriptional network revealed by genome-wide identification of BESI target genes in Arabidopsis thaliana
A brassinosteroid transcriptional network revealed by genome-wide identification of BESI target genes in Arabidopsis thaliana
复制标题
DOI：
10.1111/j.1365-313x.2010.04449.x
复制
发表时间：
2011-02-01
期刊：
影响因子：
7.2
通讯作者：
Yin, Yanhai
中科院分区：
文献类型：
--
作者：
Yu, Xiaofei;Li, Lei;Yin, Yanhai
类甾醇（BRs）是植物生长发育的重要调节因子. BR信号控制BES 1和BZR 1家族转录因子的活性。BES 1和BZR通过其调控大量靶基因的转录网络大多是未知的。通过结合染色质免疫沉淀结合拟南芥瓦片阵列（ChIP-chip）和基因表达研究，我们已经确定了1609个推定的BES 1靶基因，其中404个是由BR和/或在功能获得性bes 1-D突变体的调节。BES 1靶点有助于BR反应以及与其他激素或光信号通路的相互作用。使用精确细胞网络重建算法（ARACNe）对基因表达数据进行的计算建模表明，BES 1靶向转录因子形成了基因调控网络（GRN）。网络中许多基因的突变体在BR反应中表现出缺陷。此外，我们发现BES 1的功能，以抑制叶绿体发育，通过抑制GLK 1和GLK 2的转录因子的表达，证实了从GRN产生的假设。因此，我们的研究结果提供了一个全球性的看法BR调节基因的表达和GRN，指导未来的研究，了解BR调节植物生长。
P>Brassinosteroids (BRs) are important regulators for plant growth and development. BRs signal to control the activities of the BES1 and BZR1 family transcription factors. The transcriptional network through which BES1 and BZR regulate large number of target genes is mostly unknown. By combining chromatin immunoprecipitation coupled with Arabidopsis tiling arrays (ChIP-chip) and gene expression studies, we have identified 1609 putative BES1 target genes, 404 of which are regulated by BRs and/or in gain-of-function bes1-D mutant. BES1 targets contribute to BR responses and interactions with other hormonal or light signaling pathways. Computational modeling of gene expression data using Algorithm for the Reconstruction of Accurate Cellular Networks (ARACNe) reveals that BES1-targeted transcriptional factors form a gene regulatory network (GRN). Mutants of many genes in the network displayed defects in BR responses. Moreover, we found that BES1 functions to inhibit chloroplast development by repressing the expression of GLK1 and GLK2 transcription factors, confirming a hypothesis generated from the GRN. Our results thus provide a global view of BR regulated gene expression and a GRN that guides future studies in understanding BR-regulated plant growth.