The Cytoskeleton of Rat Aortic Smooth Muscle Cells
The Cytoskeleton of Rat Aortic Smooth Muscle Cells
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大鼠主动脉平滑肌细胞的细胞骨架
DOI：
10.1111/j.1749-6632.1986.tb54404.x
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发表时间：
1986
影响因子：
5.2
通讯作者：
G. Gabbiani
中科院分区：
文献类型：
--
作者：
G. Gabbiani
目前公认平滑肌细胞（SMC）是动脉粥样硬化斑块形成的主要因素。然而，关于中膜平滑肌细胞和动脉粥样硬化平滑肌细胞之间的形态学和/或生物化学差异知之甚少。*2*S动脉壁中S M C的细胞骨架特征最近才被阐明：”几乎所有S M C都含有由波形蛋白组成的中间丝（IF），并且许多（超过50%）含有由结蛋白组成的IF。然而，血管性平滑肌细胞的特征是普遍存在一种特殊的α-肌动蛋白I ~ o型。~ .结果表明，大部分大鼠主动脉平滑肌细胞位于内皮损伤后内膜增厚处（最常用的动脉粥样硬化斑块模型），含有仅由波形蛋白组成的IF。这表明细胞骨架元素的变化可能代表了实验性和可能的人类动脉粥样硬化过程中S M C适应的有用标志。因此，我们比较了大鼠主动脉S M C在正常条件下，实验性内膜增厚，并在体外的细胞骨架功能。我们的结果表明，内膜增厚或组织培养过程中的细胞骨架元素的修改提供了新的有用的信息，S M C的反应刺激诱导其复制和运动。
It is presently well accepted that smooth muscle cells (SMC) are the major contributor to the formation of atheromatous plaque.'-' However, little is known about morphologic and/or biochemical differences between S M C of the media and atheromatous SMC.'*2*S The cytoskeletal features of S M C in the arterial wall have been clarified only recently:"" practically all S M C contain intermediate filaments (IF) composed of vimentin and many (more than 50%) contain IF composed of desmin. However, vascular S M C are characterized by the prevalence of a special a-actin i ~ o t y p e . ~ . ~ It has been shown that the large majority of rat aortic S M C located in intimal thickening after endothelial injury (the most commonly used model for the atheromatous plaque) contain I F composed only of vimentin.' This suggests that changes in cytoskeletal elements may represent useful markers of S M C adaptation during experimental and possibly human atheromatosis. We have therefore compared the cytoskeletal features of rat aortic S M C during normal conditions, experimental intimal thickening, and in vitro Our results show that the modifications of cytoskeletal elements during intimal thickening or tissue culture furnish new useful information on S M C reaction to stimuli inducing their replication and movement.