An 'OFF-ON' fluorescent probe for specially recognize on Cys and its application in bioimaging
An 'OFF-ON' fluorescent probe for specially recognize on Cys and its application in bioimaging
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特异性识别Cys的“OFF-ON”荧光探针及其在生物成像中的应用
DOI：
10.1016/j.dyepig.2014.11.004
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发表时间：
2015-03-01
影响因子：
4.5
通讯作者：
Zhang, Yongbin
中科院分区：
文献类型：
--
作者：
Yang, Yutao;Huo, Fangjun;Zhang, Yongbin
迄今为止，针对Cys、Hcy和GSH的高选择性探针的设计仍然是一个挑战，并且迄今为止报道的实例很少。本工作合成了一种能特异识别半胱氨酸的荧光探针N-[4-甲基香豆素-7-基]马来酰亚胺。探针对Cys的特异性识别依赖于其马来酰亚胺基团、巯基本身的结构和酸性差异。此外，证明了探针通过增强荧光来检测活细胞（HepG 2细胞）中的Cys的能力。(C)2014爱思唯尔有限公司版权所有。
Up to date, the design of highly selective probes for Cys, Hcy, and GSH is still a challenge and very few examples have been reported so far. In this work, the compound, N-[4-Methylcoumarin-7-yl] maleimide was synthesized and it can specially recognize on Cys as an 'OFF ON' fluorescent probe. For specific recognition on Cys of probe depends on its maleimide group, the structural and acidic differences of biothiol itself. Furthermore, the ability of probe to detect Cys in living cells (HepG2 cells) via an enhancement of the fluorescence was proved. (C) 2014 Elsevier Ltd. All rights reserved.