Hypertonic saline inhibits airway smooth muscle contraction by inhibiting Ca2+ sensitization
Hypertonic saline inhibits airway smooth muscle contraction by inhibiting Ca2+ sensitization
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高渗盐水通过抑制Ca2+敏化抑制气道平滑肌收缩
DOI：
10.1111/1440-1681.12807
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发表时间：
2017-10-01
影响因子：
2.9
通讯作者：
Yu，Meng-Fei
中科院分区：
文献类型：
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作者：
Liu，Xiao-Cao;Wang，Qian;Yu，Meng-Fei
高渗溶液对气道平滑肌（ASM）收缩的影响及其机制尚不清楚。我们发现高渗生理盐水（HS）抑制乙酰胆碱（ACh）诱导的小鼠气管和人支气管的ASM收缩。在单个小鼠ASM细胞（ASMCs）中，ACh诱导细胞内Ca2+增加，5% NaCl进一步增强，表明HS诱导的ASM收缩抑制不是由胞质Ca2+减少介导的。Rho相关激酶（ROCK）抑制剂Y - 27632可缓解乙酰胆碱诱导的小鼠气管环预收缩。而5% NaCl诱导弛豫后，则没有这种抑制作用。此外，用5% NaCl孵育小鼠气管环可降低ACh诱导的肌球蛋白轻链20和肌球蛋白磷酸酶靶亚基1的磷酸化。这些数据表明，HS通过抑制Ca2+敏化来抑制ASM的收缩，而不是通过降低细胞内Ca2+。
The effects of hypertonic solution on airway smooth muscle (ASM) contraction and the underlying mechanisms are largely unknown. We found that hypertonic saline (HS) inhibited acetylcholine (ACh)‐induced contraction of ASM from the mouse trachea and human bronchi. In single mouse ASM cells (ASMCs), ACh induced an increase in intracellular Ca2+that was further enhanced by 5% NaCl, indicating that the HS‐induced inhibition of ASM contraction was not mediated by a decrease in cytosolic Ca2+. The Rho‐associated kinase (ROCK) inhibitor Y‐27632 relaxed ACh‐induced precontraction of mouse tracheal rings. However, such inhibition was not observed after the relaxation induced by 5% NaCl. Moreover, the incubation of mouse tracheal rings with 5% NaCl decreased ACh‐induced phosphorylation of myosin light chain 20 and myosin phosphatase target subunit 1. These data indicate that HS inhibits the contraction of ASM by inhibiting Ca2+sensitization, not by decreasing intracellular Ca2+.