LACTATE DEHYDROGENASE ISOZYMES - DISSOCIATION AND RECOMBINATION OF SUBUNITS
LACTATE DEHYDROGENASE ISOZYMES - DISSOCIATION AND RECOMBINATION OF SUBUNITS
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DOI：
10.1126/science.140.3573.1329
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发表时间：
1963-01-01
期刊：
影响因子：
56.9
通讯作者：
MARKERT, CL
中科院分区：
文献类型：
--
作者：
MARKERT, CL
牛肉组织中乳酸脱氢酶可电泳分解为五种同工酶，每种同工酶均为四聚体。这些四聚体可以通过在1 M氯化钠中冷冻解离成单体。解冻时，发生重新结合成功能性四聚体。根据电荷和氨基酸组成，存在两种单体。乳酸脱氢酶-1含有一种单体，乳酸脱氢酶-5含有另一种单体。等量的这两种同工酶的混合物，在解离和再结合之后，导致以1：4：6：4：1的预期比例产生所有五种同工酶。
Lactate dehydrogenase from beef tissues may be resolved elec-trophoretically into five isozymes each of which is a tetramer. These tetramers can be dissociated into monomers by freezing in 1M sodium chloride. On thawing, reassociation into functional tetramers occurs. On the basis of charge and amino acid composition there are two kinds of monomers. Lactate dehydrogenase-1 contains are kind of monomer and lactate dehydrogenase-5 the other kind. A mixture of equal quantities of these two isozymes, after dissociation and reassociation, leads to the production of all five isozymes in the expected proportions of 1:4:6:4:1.