Lysosomal and lipid alterations in the digestive gland of mussels, Mytilus galloprovincialis (L.) as biomarkers of environmental stress
Lysosomal and lipid alterations in the digestive gland of mussels, Mytilus galloprovincialis (L.) as biomarkers of environmental stress
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DOI：
10.1016/s0269-7491(00)00233-5
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发表时间：
2001-01-01
影响因子：
8.9
通讯作者：
Dimitriadis, VK
中科院分区：
文献类型：
--
作者：
Domouhtsidou, GP;Dimitriadis, VK
溶酶体和脂质的变化在消化腺细胞的常见贻贝贻贝galloprovincialis收集从六个站沿着Thermaikos海湾（北方希腊）进行了监测，通过1年的时间。采样贻贝的消化细胞的超微结构观察表明，较高的发病率的“颗粒状”或“黑暗”的残留机构在相对污染较轻的污染站相比。（1）使用溶酶体膜稳定性试验（2）在超微结构水平上定量消化细胞的残留体和异溶酶体的溶酶体结构变化，以及（3）通过环氧树脂切片上的X射线微量分析测定溶酶体的元素组成，评估消化细胞溶酶体系统的变化。利用组织化学树脂切片上的图像分析实现中性脂质的评价。污染最严重的站贻贝表现出减少溶酶体。膜的稳定性，更高的体积密度和直径的异溶酶体和残留体，在某些情况下，相对较高的溶酶体重金属含量相比，从污染较少的站贻贝。结果表明，溶酶体膜的稳定性和残留体的体积密度比其他生物标志物更敏感的一般应力，并可以成功地用于生物监测计划的早期预警工具。(C)2001爱思唯尔科技有限公司版权所有。
Lysosomal and lipid alterations in the digestive gland cells of the common mussel Mytilus galloprovincialis collected from six stations along Thermaikos Gulf (Northern Greece) were monitored through a 1-year period. The ultrastructural observations of the digestive cells of sampled mussels indicated a higher incidence of "granular" or "dark" residual bodies in the relatively more polluted stations compared to less polluted ones. Alterations in the lysosomal system of digestive cells were assessed (1) using the lysosomal membrane stability test (2) quantifying lysosomal structural alterations of the residual bodies and the heterolysosomes of the digestive cells at the ultrastructural level, and (3) determining the elemental composition of lysosomes by means of X-ray microanalysis on epoxy resin sections. Evaluation of neutral lipids was realized using image analysis on histochemical resin sections. Mussels from the most heavily polluted stations exhibited reduced lysosomal. membrane stability, higher volume density and diameter of heterolysosomes and residual bodies, and, in some cases, relatively higher lysosomal heavy metal content compared to mussels from less polluted stations. The results showed that the lysosomal membrane stability and the volume density of the residual bodies are more sensitive to general stress compared to the other biomarkers used, and could be used successfully as early warning tools in biomonitoring programs. (C) 2001 Elsevier Science Ltd. All rights reserved.