INTERACTION OF CERULEIN AND SECRETIN ON PANCREATIC SIZE AND COMPOSITION IN RAT
INTERACTION OF CERULEIN AND SECRETIN ON PANCREATIC SIZE AND COMPOSITION IN RAT
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DOI：
10.1152/ajpendo.1978.235.6.e714
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发表时间：
1978-01-01
影响因子：
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通讯作者：
GROSSMAN, MI
中科院分区：
文献类型：
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作者：
SOLOMON, TE;PETERSEN, H;GROSSMAN, MI
给大鼠皮下注射雨蛙肽、胰泌素或这2种肽的组合。每日3次，持续5、10或15天。雨蛙肽产生显着的剂量和时间依赖性增加胰腺重量和内容的DNA，RNA，蛋白质，淀粉酶，胰蛋白酶原。促胰液素治疗15天后，胰腺重量和RNA和脂肪酶含量显着增加。用促胰液素加雨蛙肽的组合治疗仅5天后，胰腺重量和RNA和蛋白质的含量增加了一倍以上，胰蛋白酶原含量增加了5倍以上。比较5天、10天和15天的平均值，分泌素加雨蛙肽组合的wt、蛋白质和胰蛋白酶原的增加显著大于单独给予的肽的作用的总和。使用DNA含量的增加作为增生的指标，并使用胰腺重量、RNA含量和蛋白质含量与DNA含量的比率的增加作为肥大的指标，雨蛙肽同时引起大鼠胰腺腺泡细胞的增生和肥大。分泌素显着增强雨蛙素的肥大作用，但不改变其增生作用。
Rats were given injections of caerulein, secretin or a combination of these 2 peptides s.c. 3 times daily for 5, 10, or 15 days. Caerulein produced significant dose- and time-dependent increases in pancreatic wt and content of DNA, RNA, protein, amylase, and trypsinogen. Secretin produced significant increases in pancreatic wt and content of RNA and lipase after 15 days of treatment. After only 5 days of treatment with a combination of secretin plus caerulein, pancreatic wt and content of RNA and protein more than doubled, and trypsinogen content increased more than 5-fold. Comparing the averages across the 5-, 10-, and 15-day values, increases in wt, protein, and trypsinogen with the combination of secretin plus caerulein were significantly greater than the sum of the effects of the peptides given singly. Using increase in DNA content as an index of hyperplasia and increases in the ratios of pancreatic wt, RNA content, and protein content to DNA content as indices of hypertrophy, caerulein produced both hyperplasia and hypertrophy of rat pancreatic acinar cells. Secretin markedly augmented the hypertrophic action of caerulein but did not alter its hyperplastic action.