Emergence and serovar transition of Vibrio parahaemolyticus pandemic strains isolated during a diarrhea outbreak in Vietnam between 1997 and 1999
Emergence and serovar transition of Vibrio parahaemolyticus pandemic strains isolated during a diarrhea outbreak in Vietnam between 1997 and 1999
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DOI：
10.1111/j.1348-0421.2004.tb03513.x
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发表时间：
2004-01-01
影响因子：
2.6
通讯作者：
Nishibuchi, M
中科院分区：
文献类型：
--
作者：
Chowdhury, A;Ishibashi, M;Nishibuchi, M
我们在1997年至1999年期间对越南庆和省腹泻患者进行的调查中分离出523株副溶血性弧菌。49%的菌株被判定属于1996年左右出现并传播到许多国家的大流行菌株。这些菌株在GS-PCR检测中呈阳性，并携带tdh基因。F237噬菌体基因组的ORF 8（大流行克隆的可能标记）在10%的这些菌株中不存在。11 O：K血清型中检测到的流行株和代表所有11个血清型的流行株的菌株被证明是密切相关的，无论OPF 8基因型使用任意引物PCR和脉冲场凝胶电泳分析。很明显，在大流行毒株中发生了主要血清型的转变，以1997年出现的O3：K6、1998年出现的O 4：K68以及1998年和1999年出现的O 1：K25为代表。
We characterized 523 Vibrio parahaemolyticus strains isolated during a survey of diarrhea patients in Khanh Hoa province, Vietnam between 1997 and 1999. Forty-nine percent of the strains were judged to belong to the pandemic strains that emerged around 1996 and spread to many countries. These strains were positive in the GS-PCR assay and carried the tdh gene. The ORF8 of the f237 phage genome, a possible marker of the pandemic clone, was absent in 10% of these strains. Eleven O:K serovars were detected among the pandemic strains and the strains representing all 11 serovars of pandemic strains were shown to be closely related regardless of the OPF8 genotype using arbitrarily primed PCR and pulsed field gel electrophoresis analyses. It was clear that a transition of major serovars occurred among the pandemic strains represented by the emergence of O3:K6 in 1997, O4:K68 in 1998, and O1:K25 in 1998 and 1999.