Complete nucleotide sequence of the type isolate of Cowpea mild mottle virus from Ghana
Complete nucleotide sequence of the type isolate of Cowpea mild mottle virus from Ghana
复制标题
加纳豇豆轻斑驳病毒典型分离株的完整核苷酸序列
作者：
W. Menzel;S. Winter;H. Vetten
豇豆轻斑驳病毒（Cowpeamildmottlevirus，CPMMV）是引起豇豆（VignaunguiculataL.）1973年[5]，广泛分布于西非加纳东部地区。从那时起，它被描述为自然感染其他豆科作物，如花生（Arachis hypogaea L.），大豆（Glycine max L.）和菜豆（Phaseolus vulgaris L.）[19]第10段。CPMMV症状被认为是季节性变化的。在花生上，它可能导致系统性褪绿、褪绿环、卷叶或脉坏死，在大豆上，已知它会导致褪绿、脉花叶和顶端坏死或其他畸形[5，12，13，15]。CPMMV具有曲折的丝状颗粒（约100 μ m）。长度为650 nm），类似于卡拉病毒属的成员。与绝大多数由蚜虫传播的香石竹病毒不同，CPMMV是由烟粉虱Bemisia tabaci G. [12[17]也被归为卡拉病毒属[2]。它与蚜虫传播的香石竹病毒的不同之处还在于其在宿主植物中的细胞内发生[6]。与其他感染豆科成员的香石竹病毒，如豌豆条纹病毒（PeSV）和红三叶草脉花叶病毒（RCVMV）一致，CPMMV被认为是在其许多豆科宿主中种子传播的，即使有相互矛盾的报道[5，11，12]。CPMMV的实验寄主范围包括几个科的植物物种，这些植物物种在接种/感染后主要表现出褪绿的局部病变或全身斑驳。自非洲首次报告以来[5，20]，该病毒已在印度[13]，泰国[12]和南美洲[8，19]发现。番茄（Solanum lycopersicum L.）据报道，以色列[3，7，14]。番茄苍白褪绿病菌株和茄子（Solanum melongena L.）来自约旦的分离物与来自印度和西非的豆科分离物引起轻度叶镶嵌，表明来自茄科宿主的分离物与豆科分离物非常相似，但明显不同[15]。第一个发表的关于CPMMV的核苷酸序列信息（在GenBank中不可用）是在加纳东部地区收集的西非豇豆分离物的30-末端0.9-kb片段[4]。它是使用通用引物产生的香石竹病毒，并支持分配CPMMV属香石竹病毒。从那时起，六个30-末端部分序列覆盖0.9 kb [9]至2.5 kb [18]已提交给GenBank。在本文中，我们报告了第一个完整的基因组序列的CPMMV分离物，这可能是相同的（类型）分离物的部分测序Badge等。
Cowpea mild mottle virus (CPMMV) was first reported to cause systemic mottling, chlorotic blotches and leaf malformations in cowpea (Vigna unguiculata L.) in 1973 [5] and to be widespread in the Eastern Region of Ghana, West Africa. Since then, it has been described to naturally infect other leguminous crops such as peanut (Arachis hypogaea L.), soybean (Glycine max L.) and common bean (Phaseolus vulgaris L.) [19]. CPMMV symptoms are thought to vary seasonally. On peanut, it might cause systemic chlorosis, chlorotic rings, leaf rolling or veinal necrosis, and on soybean, it is known to cause chlorosis, vein mosaic, and apical necrosis or other malformations [5, 12, 13, 15]. CPMMV has flexuous filamentous particles (approx. 650 nm in length) resembling those of members of the genus Carlavirus. In contrast to the vast majority of carlaviruses, which are aphid-transmitted, CPMMV is transmitted in a non-persistent manner by the whitefly Bemisia tabaci G. [12, 17] and has also been assigned to the genus Carlavirus [2]. It differs from aphid-borne carlaviruses also in its intracellular occurrence in its host plants [6]. In accordance with other carlaviruses infecting members of the family Fabaceae, such as pea streak virus (PeSV) and red clover vein mosaic virus (RCVMV), CPMMV is thought to be seed-borne in a number of its leguminous hosts, even if there have been contradictory reports [5, 11, 12]. The experimental host range of CPMMV includes plant species from several families, which mainly display chlorotic local lesions or systemic mottle upon inoculation/ infection. Since the initial reports from Africa [5, 20], the virus has been discovered in India [13], Thailand [12] and South America [8, 19]. A distinct isolate responsible for pale chlorosis disease in tomato (Solanum lycopersicum L.) was reported from Israel [3, 7, 14]. Comparisons of a tomato pale chlorosis isolate and an eggplant (Solanum melongena L.) isolate from Jordan that causes a mild leaf mosaic with legume isolates from India and West Africa showed that the isolates from solanaceous hosts are very similar to, but clearly distinct from, the legume isolates [15]. The first published nucleotide sequence information about CPMMV (not available in GenBank) was a 30-terminal 0.9-kb fragment of a West African cowpea isolate collected in the eastern region of Ghana [4]. It was generated using a universal primer for carlaviruses and supported the assignment of CPMMV to the genus Carlavirus. Since then, six 30-terminal partial sequences covering 0.9 kb [9] to 2.5 kb [18] have been submitted to GenBank. In this paper, we report the first complete genome sequence of a CPMMV isolate, which is probably the same (type) isolate as that partially sequenced by Badge et al. [4].