Sex differences in hepatic monooxygenases in winter flounder (Pseudopleuronectes americanus) and scup (Stenotomus chrysops) and regulation of P450 forms by estradiol.
Sex differences in hepatic monooxygenases in winter flounder (Pseudopleuronectes americanus) and scup (Stenotomus chrysops) and regulation of P450 forms by estradiol.
复制标题
冬鲽 (Pseudopleuronectes americanus) 和 scup (Stenotomus chrysops) 肝脏单加氧酶的性别差异以及雌二醇对 P450 形式的调节。
DOI：
10.1002/jez.1402590308
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Stegeman,JJ
中科院分区：
文献类型：
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作者：
Gray,ES;Woodin,BR;Stegeman,JJ
关于硬骨鱼肝脏细胞色素P450单加氧酶的内源性调节的细节，以及这种调节在鱼类中普遍存在的特征，人们知之甚少。据报道，性腺成熟的雌性冬季比目鱼(Pseudopleuronectes Americanus)的微粒体细胞色素P450和B5以及NADPH细胞色素C还原酶的水平比雄性低几倍(Stegeman和Woodin(‘84)Mar.Environ)。研究结果，14：422-425)。这些强烈的性别差异促使人们对冬季比目鱼肝脏中P450的调节进行了更详细的研究，并与另一种海洋硬骨鱼Scup(Stenotomus Chrysops)的性别差异进行了比较。乙氧基间苯二酚O-脱乙基酶(EROD)活性/nmolP450在性腺成熟雌体中低于雄体。用单抗1-12-3对P450E(EROD催化剂)的免疫印迹分析表明，P450E对应物在两种雌虫中的含量也很少。性腺成熟雌体的氨基比林N-脱甲基酶(APND)和睾酮6-β-羟基酶(6-β-OHase)活性在性腺成熟的雌体中高于成熟雄体，在Scup中未见差异。针对Scup450A的多克隆抗体被证明可以在许多硬骨鱼中检测到蛋白质。成鱼体内抗P450A交叉反应蛋白水平高于雄性，但与6-β-OHase活性一样，该蛋白的含量在牙鲆中没有性别差异。雌二醇处理降低了每毫克蛋白中EROD、APND、6-β-OHase和雌二醇2-OHase活性，但APND和6-β-OHase活性/nmolP450没有变化。因此，E2促进了一些肝脏P450催化活性的普遍下降，但在实现性别差异的过程中，也有对P450E对应物的特定调节，可能还有对6β-OHase(P450A？)的调节。其他因素，时间或激素，可以改变E2处理的效果，并可能有助于对自然成熟鱼类中P450形式的特定调节，以及这种调节中的物种差异。
Details concerning the endogenous regulation of hepatic cytochrome P450 mono‐oxygenases in teleosts, and the features of this regulation common among fish species, are poorly known. Gonadally mature female winter flounder (Pseudopleuronectes americanus) have been reported to have severalfold lower levels of microsomal cytochromes P450 and b5and NADPH cytochrome c reductase than do males (Stegeman and Woodin ('84) Mar. Environ. Res., 14:422–425). These strong sex differences prompted more detailed study of P450 regulation in winter flounder liver, and a comparison with sex differences in another marine teleost, scup (Stenotomus chrysops). Ethoxyresorufin O‐deethylase (EROD) activity/nmol P450 was less in gonadally mature females than in males of both species. Immunoblot analysis with MAb 1‐12‐3 to P450E (the EROD catalyst) showed that the content of P450E counterpart was also much less in females of both species. Aminopyrine N‐demethylase (APND) and testosterone 6β‐hydroxylase (6β‐OHase) activities per nmol P450 were higher in gonadally mature female than in mature male flounder, differences not seen in scup. Polyclonal antibodies to scup P450A were shown to detect proteins in a number of teleosts. The levels of anti‐P450A cross‐reacting protein were greater in mature female than in male flounder, but as with 6β‐OHase activity, the content of this protein was not sexually differentiated in scup. Estradiol treatment of winter flounder depressed the rates of EROD, APND, 6β‐OHase, and estradiol 2‐OHase activities per mg protein, but APND and 6β‐OHase activities per nmol P450 were unchanged. Thus, E2promotes general decreases in some hepatic P450‐catalyzed activities, but in achieving sex differences there is also specific regulation of the P450E counterpart, and possibly of the 6β‐OHase (P450A?). Other factors, temporal or hormonal, can modify the effect of E2treatment, and may contribute to the specific regulation of P450 forms in naturally maturing fish, and to species differences in this regulation.