Microbiome of permeable sandy substrate in headwater river is shaped by water chemistry rather than grain size and heterogeneity
Microbiome of permeable sandy substrate in headwater river is shaped by water chemistry rather than grain size and heterogeneity
复制标题
源头河流渗透性沙质基质的微生物组是由水化学而不是颗粒大小和异质性决定的
DOI：
10.1016/j.scitotenv.2021.146552
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发表时间：
2021
影响因子：
9.8
通讯作者：
Zhang Quanfa
中科院分区：
文献类型：
--
作者：
Wang Weibo;Hu Mingming;Shu Xiao;Li Hua;Qi Wenhua;Yang Yuyi;Zhang Quanfa
土壤和沉积物中的微生物群落库执行许多生态功能，并提供许多生态系统服务。其多样性和群落结构可能与不同的粒度和异质性有关。然而，这些结论大多来自于在陆地土壤、不透水的海洋和淡水沉积物（基质）中进行的研究。这些结论是否适用于渗透性基质，特别是河源河流生态系统，还有待观察。为了解决这一问题，田间试验的目的是评估颗粒中值大小和分布异质性和微生物多样性和群落结构之间的联系。以华中地区某水源河为研究对象，采用梯度粒径和异质性的渗透性基质进行接种，3个月后采用高通量测序法对总微生物群落和3种净水剂群落的多样性和群落组成进行了研究。总的微生物群落进行了测序的16S rRNA，分类多样性的标记。使用三种功能基因标记：nirK、nirS和nosZ对三个细菌群落进行测序。结果表明，尽管粒径和异质性对基质养分浓度有正向影响，但土壤微生物群落和3种微生物群落的多样性和群落结构主要由水化学因素决定，而不是由粒径和粒径分布的异质性决定。与总微生物群落相比，反硝化功能群具有更多独特的物种比例，表明功能基因比16SrRNA基因对环境变化更敏感。我们的研究填补了了解源头河流中可渗透沉积物中微生物群落的空白，并强调了粒度和mm尺度异质性在塑造微生物组多样性和结构方面的重要性较小。
The reservoir of microbial communities within the soil and the sediment performs many ecological functions and offers many ecosystem services. It has been suggested that its diversity and community structure could be explained by different grain size and heterogeneity. However, most of these conclusions come from studies conducted in terrestrial soil, impermeable marine and freshwater sediment (substrate). It remains to be seen whether these conclusions hold true in permeable substrate, especially in headwater river ecosystems. To address this, a field experiment was aimed to evaluate the link between grain median size and distribution heterogeneity and microbial diversity and community structure. Permeable substrate with gradient grain sizes and heterogeneities was inoculated in a headwater river in central China, while the diversity and community composition of the total microbial community and three denitrifier communities were investigated by high throughput sequencing three months later. The total microbial community was sequenced by 16S rRNA, a marker for taxonomic diversity. Three denitrifier communities were sequenced using three functional gene markers: nirK, nirS, and nosZ. The result showed that both the diversity and community structure of the total microbial community and three denitrifier communities were determined by water chemistry rather than grain size and size distribution heterogeneity, although grain size and heterogeneity positively influenced the nutrient concentrations of the substrate. Compared to the total microbial community, denitrification functional groups had more unique species proportions, indicating that functional genes were more sensitive to environmental change than the 16S rRNA gene. Our study fills a gap in understanding microbial communities in permeable sediment in a headwater river and highlights the less importance of grain size and heterogeneity on mm-scale in shaping the diversity and structure of microbiome.