Design and application of a biosensor for monitoring toxicity of compounds to eukaryotes
Design and application of a biosensor for monitoring toxicity of compounds to eukaryotes
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DOI：
10.1128/aem.66.4.1676-1679.2000
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发表时间：
2000-04-01
影响因子：
4.4
通讯作者：
Glover, LA
中科院分区：
文献类型：
--
作者：
Hollis, RP;Killham, K;Glover, LA
在这里，我们描述了一种替代方法，目前使用的细胞毒性分析，通过应用真核微生物生物传感器。酵母菌经过基因改造以表达萤火虫荧光素酶，产生生物发光酵母菌株，任何干扰细胞代谢的有毒化学物质的存在都会导致生物发光的定量减少。在这项研究中，它被证明发光酵母菌株的感官化学品已知是有毒的真核生物样品评估为无毒的原核生物传感器。由于S.酿酒酵母细胞增强稳定性并保护免受极端pH、溶剂暴露和渗透压休克的影响，这些固有性质被利用来产生生物传感器，该生物传感器应当在极端条件下检测宽范围的有机和无机毒素。
Here we describe an alternative approach to currently used cytotoxicity analyses through applying eukaryotic microbial biosensors. The yeast Saccharomyces cererisiae was genetically modified to express firefly luciferase, generating a bioluminescent yeast strain, The presence of any toxic chemical that interfered with the cells' metabolism resulted in a quantitative decrease in bioluminescence. In this study, it was demonstrated that the Luminescent yeast strain senses chemicals known to be toxic to eukaryotes in samples assessed as nontoxic by prokaryotic biosensors. As the cell wall and adaptive mechanisms of S. cerevisiae cells enhance stability and protect from extremes of pH, solvent exposure, and osmotic shock, these inherent properties were exploited to generate a biosensor that should detect a wide range of both organic and inorganic toxins under extreme conditions.