A new mode for highly sensitive and specific detection of DNA based on exonuclease III-assisted target recycling amplification and mismatched catalytic hairpin assembly
A new mode for highly sensitive and specific detection of DNA based on exonuclease III-assisted target recycling amplification and mismatched catalytic hairpin assembly
复制标题
基于核酸外切酶III辅助靶标循环扩增和错配催化发夹组装的DNA高灵敏特异性检测新模式
DOI：
10.1039/c5cc00385g
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发表时间：
2015-01-01
影响因子：
4.9
通讯作者：
Ding, Shijia
中科院分区：
文献类型：
--
作者：
Tao, Chenyang;Yan, Yurong;Ding, Shijia
通过将核酸外切酶III辅助的靶循环扩增与错配催化发夹组装相结合，开发了一种新型的电化学传感策略，用于DNA的高灵敏度和特异性检测。该方法具有较高的灵敏度和选择性，动态响应范围为100 fM至5 nM。
A novel electrochemical sensing strategy was developed for highly sensitive and specific detection of DNA by combining the exonuclease III-assisted target recycling amplification with mismatched catalytic hairpin assembly. The proposed method showed high sensitivity and selectivity with a dynamic response range from 100 fM to 5 nM.