Predominant expression and activity of vacuolar H+-ATPases in the mixed segment of the wood-feeding termite Nasutitermes takasagoensis
Predominant expression and activity of vacuolar H+-ATPases in the mixed segment of the wood-feeding termite Nasutitermes takasagoensis
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食木白蚁 Nasutitermes takasagoensis 混合体液泡 H-ATP 酶的主要表达和活性
DOI：
10.1016/j.jinsphys.2015.04.012
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发表时间：
2015
影响因子：
2.2
通讯作者：
G.
中科院分区：
文献类型：
--
作者：
Kumara;R. P.;Saitoh;S.;Aoyama;H.;Shinzato;N. & Tokuda;G.
混合肠段是白蚁肠道的一个独特部分，仅存在于白蚁的最顶端谱系中，由重叠的肠系膜和直肠上皮复合体组成。尽管其结构独特，但混合节的生理功能研究很少。我们进行了转录组分析，以确定功能酶的混合段的木材喂养的高砂白蚁Nasutitermes。我们对混合段的转录本（4563个isotigs）进行了测序，并将其与中肠（4813个isotigs）和第一直肠段（3629个isotigs）的转录本进行了比较。我们发现液泡H+-ATP酶（V-ATP酶）亚基在混合段中占优势，这一点通过RT-qPCR分析得到证实。V-ATP酶在三种组织中的活性与表达模式基本一致，表明V-ATP酶是白蚁混合节中的优势酶。结果证实了混合段作为转运上皮的作用。
The mixed segment is a unique part of the gut present only in the most apical lineage of termites and consists of a complex of overlapping mesenteric and proctodeal epithelia. In spite of its unique structure, the physiological functions of the mixed segment have been poorly studied. We performed transcriptome analysis to identify functional enzymes acting in the mixed segment of the wood-feeding higher termiteNasutitermes takasagoensis. We sequenced the transcripts (4563 isotigs) of the mixed segment and compared them with those of the midgut (4813 isotigs) and the first proctodeal segment (3629 isotigs). We found that vacuolar H+-ATPase (V-ATPase) subunits were predominant in the mixed segment, which was confirmed by RT-qPCR analysis. The V-ATPase activity in these three tissues was in a good agreement with the expression patterns, suggesting that V-ATPase is a prevalent enzyme in the mixed segment of the termites. The results confirmed the proposed role of the mixed segment as a transporting epithelium.