Metabolic profiling of radioreceptor-assayable opioid peptides in a human pituitary ACTH-secreting tumor.
Metabolic profiling of radioreceptor-assayable opioid peptides in a human pituitary ACTH-secreting tumor.
复制标题
人垂体 ACTH 分泌肿瘤中放射性受体可测定的阿片肽的代谢分析。
DOI：
10.1016/0024-3205(85)90225-5
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发表时间：
1985
期刊：
影响因子：
6.1
通讯作者：
Sacks,H
中科院分区：
文献类型：
--
作者：
Desiderio,DM;Cezayirli,RC;Fridland,G;Robertson,JT;Sacks,H
介绍了从分泌ACTH的人垂体瘤的匀浆中获得的富含多肽的部分中的内源性阿片肽的分布。用梯度反相高效液相色谱法将混合物分离成多肽成分。一种阿片受体制剂用于放射受体分析，乙吗啡是一种相对非特异性的配体，由于它与MU、Sigma和Delta受体相互作用而被用作筛选物，作为高效液相检测器，以检测源自前脑啡肽A和POMC的一系列多肽。
The profile of endogenous opioid peptides in the peptide-rich fraction obtained from a homogenate of an ACTH-secreting human pituitary tumor is presented. Gradient RP-HPLC is used to separate the mixture into peptide constituents. A preparation of opioid receptors is used in a radioreceptor assay with ethorphine - a relatively non-specific ligand that is used as a screen because it interacts with mu, sigma, and delta receptors - as the HPLC detector to detect a range of peptides that derive from proenkephalin A and POMC.