SUBSURFACE IMAGING OF LIVING SKIN WITH OPTICAL COHERENCE MICROSCOPY
SUBSURFACE IMAGING OF LIVING SKIN WITH OPTICAL COHERENCE MICROSCOPY
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DOI：
10.1159/000246523
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发表时间：
1995-01-01
期刊：
影响因子：
3.4
通讯作者：
BONNER, RF
中科院分区：
文献类型：
--
作者：
SCHMITT, JM;YADLOWSKY, MJ;BONNER, RF
背景：一种新型的显微镜已经被开发用于无创地获取活体皮肤的横截面图像。它利用宽带光源的短时间相干性来抑制散射光。由于该显微镜仍处于早期开发阶段，其作为皮肤科诊断工具的潜力尚未确定。目的：探讨光学相干显微镜在皮肤科的应用前景。其目的是研究皮肤中的结构，这些结构可以在不染色或使用复杂的图像处理方法的情况下看到。方法：组装一个原型光纤显微镜，使用1,300 nm的发光二极管作为光源。扫描从食指和前臂的皮肤上获得。根据正常健康皮肤的解剖学知识识别表面下结构。结果如下：位于皮肤表面下深达1 mm的结构可以在轴向和横向维度上以约10 μ m的分辨率成像。在垂直于皮肤表面的光学切片中，容易观察到表皮脊的轮廓和表皮与真皮之间的边界。结论：这项研究的结果表明，光学相干显微镜可能有价值的情况下，亚细胞的细节可视化的诊断工具是不需要的。显微镜的分辨率、对比度和扫描速度有待提高。
Background: A new type of microscope has been developed for acquiring cross-sectional images of living skin noninvasively. It takes advantage of the short temporal coherence of a broad-band light source to reject scattered light. Because this microscope is still in an early stage of development, its potential as a diagnostic tool in dermatology has not yet been determined. Objective: This study was designed to explore potential applications of optical coherence microscopy in dermatology. The aim was to investigate the structures in skin that can be seen without staining or using sophisticated image-processing methods. Methods: A prototype fiberoptic microscope was assembled that uses a 1,300-nm light-emitting diode as a light source. Scans were obtained from the skin on the index finger and forearm. Subsurface structures were identified based on knowledge of the anatomy of normal healthy skin. Results: Structures located as deep as 1 mm below the surface of the skin could be imaged with a resolution of about 10 mu m in the axial and lateral dimensions. In optical slices taken perpendicular to the skin surface, the contours of the epidermal ridges and the boundary between the epidermis and dermis were readily observed. Conclusions: The results of this study suggest that an optical coherence microscope may have value as a diagnostic tool for cases in which visualization of subcellular details is not required. The resolution, contrast and scanning speed of the microscope need to be improved.