Identification of the Fcα/μR isoform specifically expressed in the kidney tubules
Identification of the Fcα/μR isoform specifically expressed in the kidney tubules
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DOI：
10.1016/j.molimm.2008.10.002
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发表时间：
2009-02-01
影响因子：
3.6
通讯作者：
Shibuya, Akira
中科院分区：
文献类型：
--
作者：
Kurita, Naoki;Honda, Shin-ichiro;Shibuya, Akira
Fc α/μ R不仅在淋巴器官中表达，而且在非淋巴器官（包括肾脏）中表达。然而，Fc α/μ R的分子和功能特征，特别是在非淋巴器官中表达的，仍然不清楚。在此，我们在C57 BL/6 J背景下的鼠肾脏中鉴定了Fc α/μ R的同种型。肾脏仅表达同种型，其在其他淋巴和非淋巴器官中不表达，并且该同种型结合伊加和IgM。免疫组织化学分析表明，Fc α/μ R亚型表达在肾小管上皮细胞，但不是在肾小球。这证实了分离的肾小管上皮细胞的流式细胞术分析和RT-PCR分析，分别切除肾小球和肾小管区域的激光显微切割系统。这些结果表明，Fc α/μ R可能不参与伊加肾病肾小球系膜细胞中伊加沉积。相反，它可能在肾小管区域的免疫中发挥重要作用。(C)2008爱思唯尔有限公司版权所有。
Fc alpha/mu R is expressed not only in lymphoid, but also in non-lymphoid organs, including kidney. However, molecular and functional characteristics of Fc alpha/mu R, particularly expressed in non-lymphoid organs, have remained unclear. Here we identified an isoform of Fc alpha/mu R in the murine kidney on the C57BL/6J background. The kidney expressed only the isoform, which was not expressed in other lymphoid and non-lymphoid organs, and this isoform binds both IgA and IgM. Immunohistochemical analyses suggested that the Fc alpha/mu R isoform was expressed in the tubular epithelial cells, but not in the glomeruli. This was confirmed by flow cytometry analysis of isolated tubular epithelial cells and by RT-PCR analyses using the separately excised glomerular and tubular regions by the laser microdissection system. These results suggest that Fc alpha/mu R may not be involved in IgA deposition in glomerular mesangium cells in IgA nephropathy. Rather, it may play an important role in immunity in the renal tubular regions. (C) 2008 Elsevier Ltd. All rights reserved.