Dopaminergic neurons associate with blood vessels in neural transplants.
Dopaminergic neurons associate with blood vessels in neural transplants.
复制标题
多巴胺能神经元与神经移植中的血管相关。
DOI：
10.1016/s0014-4886(03)00336-4
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Pidel,Ann
中科院分区：
文献类型：
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作者：
Casper,Diana;Finkelstein,Eric;Goldstein,IraM;Palencia,David;Yunger,Yaacov;Pidel,Ann
神经移植对于导致局灶性神经变性的疾病是一种有吸引力的策略，例如帕金森病，其中中脑黑质中的多巴胺能神经元存在选择性损失。然而，其应用的一个主要缺点是供体多巴胺能神经元的存活率很低。虽然神经元可能依赖于通过扩散或建立功能性血管连接递送的宿主来源物质，但每种递送机制的相对重要性尚不清楚。我们研究了胚胎中脑组织移植的地形图，并描述了移植的多巴胺能神经元，宿主脑和生长中的血管之间的空间关系。结果表明，移植血管化与脑血管化的发育模式有共同的特征。此外，多巴胺能神经元的地形分布反映了它们与宿主脑的接近程度以及它们与血管成分的距离。区域分析显示，大多数多巴胺能神经元在移植后1周被发现在宿主-移植物界面或附近。最近邻分析表明，在同一时间点，多巴胺能神经元的下降指数梯度作为其与血管的距离的函数。随着时间的推移，这些模式变得更加明显。结果表明，血管化的速度和模式可能是多巴胺能神经元长期存活的重要决定因素。
Neural transplantation is an attractive strategy for diseases that result in focal neurodegeneration such as Parkinson's disease, where there is a selective loss of dopaminergic neurons in the substantia nigra of the midbrain. A major drawback to its application, however, is the poor survival of donor dopaminergic neurons. While neurons probably depend on host-derived substances delivered by either diffusion or the establishment of functional vascular connections, the relative importance of each delivery mechanism is not known. We investigated the topography of transplants of embryonic mesencephalic tissue and describe the spatial relationships between transplanted dopaminergic neurons, the host brain, and in-growing blood vessels. Results indicate that transplant vascularization shares features with developmental patterns of brain vascularization. Moreover, the topographical distribution of dopaminergic neurons reflected their proximity to the host brain as well as their distance from vascular elements. Zonal analysis revealed that the majority of dopaminergic neurons were found at or near the host–transplant interface at 1 week after transplantation. Nearest neighbor analysis demonstrated a descending exponential gradient of dopaminergic neurons as a function of their distance from vessels at the same time point. These patterns became more marked with time. Results suggest that rates and patterns of vascularization may be important determinants in the long-term survival of dopaminergic neurons.