Integrated analysis of long noncoding RNA and mRNA expression profile in children with obesity by microarray analysis.
Integrated analysis of long noncoding RNA and mRNA expression profile in children with obesity by microarray analysis.
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通过微阵列分析整合分析肥胖儿童的长非编码RNA和mRNA表达谱
DOI：
10.1038/s41598-018-27113-w
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发表时间：
2018-06-08
影响因子：
4.6
通讯作者：
Xiao Y
中科院分区：
文献类型：
--
作者：
Liu Y;Ji Y;Li M;Wang M;Yi X;Yin C;Wang S;Zhang M;Zhao Z;Xiao Y
长链非编码RNA（lncRNA）在脂肪组织功能和能量代谢稳态中具有重要作用，其异常可能导致肥胖。为了研究lncRNA是否参与儿童肥胖，我们研究了肥胖儿童与非肥胖儿童相比lncRNA的差异表达谱。共鉴定出1268个差异表达的lncRNA和1085个差异表达的mRNA。基因本体论（GO）和通路分析表明，这些lncRNA参与多种生物学过程，包括炎症反应、脂质代谢、破骨细胞分化和脂肪酸代谢。此外，lncRNA-mRNA共表达网络和蛋白质-蛋白质相互作用（PPI）网络的构建，以确定基于微阵列表达谱的枢纽调控lncRNA和基因。本研究首次发现了肥胖儿童中差异表达的lncRNA的表达谱，提示hub lncRNA RP 11 -20G13.3可抑制前脂肪细胞的脂肪生成，这将有助于寻找儿童肥胖症的新的诊断和治疗策略。
Long noncoding RNAs (lncRNAs) have an important role in adipose tissue function and energy metabolism homeostasis, and abnormalities may lead to obesity. To investigate whether lncRNAs are involved in childhood obesity, we investigated the differential expression profile of lncRNAs in obese children compared with non-obese children. A total number of 1268 differentially expressed lncRNAs and 1085 differentially expressed mRNAs were identified. Gene Ontology (GO) and pathway analysis revealed that these lncRNAs were involved in varied biological processes, including the inflammatory response, lipid metabolic process, osteoclast differentiation and fatty acid metabolism. In addition, the lncRNA-mRNA co-expression network and the protein-protein interaction (PPI) network were constructed to identify hub regulatory lncRNAs and genes based on the microarray expression profiles. This study for the first time identifies an expression profile of differentially expressed lncRNAs in obese children and indicated hub lncRNA RP11-20G13.3 attenuated adipogenesis of preadipocytes, which is conducive to the search for new diagnostic and therapeutic strategies of childhood obesity.
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长期的非编码RNA可以充当高脂饮食诱发的肥胖症的父亲遗传的媒介。
影响因子：
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作者：
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影响因子：
30.8
作者：
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通讯作者：
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作者：
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通讯作者：
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