Two populations of sympathetic neurons project selectively to mesenteric artery or vein
Two populations of sympathetic neurons project selectively to mesenteric artery or vein
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DOI：
10.1152/ajpheart.1999.276.4.h1263
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发表时间：
1999-04-01
影响因子：
4.8
通讯作者：
Travagli, RA
中科院分区：
文献类型：
--
作者：
Browning, KN;Zheng, ZL;Travagli, RA
本研究的目的是确定投射到肠系膜动脉的肠系膜下神经节（IMG）的交感神经元是否可以通过其定位、神经化学表型和电生理特性与投射到肠系膜静脉的神经元区分开来。在体外完整血管img制备中，在肠系膜下动脉（IMA）或静脉（IMV）内注射氟金或罗丹明珠后，对神经元进行标记。投射到IMV的神经元的体胞位于IMG的中央部分，而投射到IMV的神经元的体胞位于更外围。标记的神经元都没有被双重标记。18.9%的IMA神经元有神经肽Y免疫反应性，而IMV神经元无。利用全细胞膜片钳记录对鉴定的神经元进行解离和表征。直接去极化后，所有标记的动脉和静脉神经元都被归类为强直性放电，而未标记的神经元只有40%被归类为强直性放电；其余60%未标记的神经元为相放电。结果表明，投射到肠系膜动脉的IMG神经元与投射到肠系膜静脉的神经元不同。
The objective of this study was to determine whether sympathetic neurons of the inferior mesenteric ganglion (IMG) projecting to mesenteric arteries could be distinguished by their localization, neurochemical phenotype, and electrophysiological properties from neurons projecting to mesenteric veins. In an in vitro intact vasculature-IMG preparation, neurons were labeled following intraluminal injection of Fluoro-Gold or rhodamine beads into the inferior mesenteric artery (IMA) or vein (IMV). The somata of neurons projecting to IMA were localized in the central part of the IMG, whereas those projecting to IMV were localized more peripherally. None of the labeled neurons was doubly labeled. Neuropeptide Y immunoreactivity was found in 18.9% of neurons innervating the IMA, but not in neurons innervating the IMV. Identified neurons were dissociated and characterized using whole cell patch-clamp recording. After direct soma depolarization, all of the labeled arterial and venous neurons were classified as tonic firing, compared with only 40% of unlabeled neurons; the remaining 60% of unlabeled neurons were phasic firing. The results indicate that IMG neurons projecting to mesenteric arteries are distinct from neurons projecting to mesenteric veins.