MOLECULAR-CLONING OF THE ALPHA-1 SUBUNIT OF AN OMEGA-CONOTOXIN-SENSITIVE CALCIUM-CHANNEL
MOLECULAR-CLONING OF THE ALPHA-1 SUBUNIT OF AN OMEGA-CONOTOXIN-SENSITIVE CALCIUM-CHANNEL
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DOI：
10.1073/pnas.89.11.5058
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发表时间：
1992-06-01
影响因子：
11.1
通讯作者：
SNUTCH, TP
中科院分区：
文献类型：
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作者：
DUBEL, SJ;STARR, TVB;SNUTCH, TP
在脊椎动物细胞中描述的四种主要类型的Ca通道（指定为T、L、N和P）中，N型Ca通道是独特的，因为它们特异性地在神经元中发现，与神经递质释放的控制相关，并且被ω-芋螺毒素（从海洋蜗牛Conus geographus分离的神经肽毒素）阻断。分离出一组重叠的cDNA克隆，发现其编码Ca通道α-1亚基，命名为rbB-I。针对rbB-I序列肽产生的多克隆抗血清选择性免疫沉淀标记大鼠前脑膜的高亲和力I-125标记的ω-芋螺毒素结合位点。PCR分析表明，与N型钙通道一样，rbB-1的表达仅限于神经系统和神经源性细胞系。该脑Ca通道可介导许多突触处神经递质释放所需的ω-芋螺毒素敏感性Ca内流。
Of the four major types of Ca channel described in vertebrate cells (designated T, L, N, and P), N-type Ca channels are unique in that they are found specifically in neurons, have been correlated with control of neurotransmitter release, and are blocked by omega-conotoxin, a neuropeptide toxin isolated from the marine snail Conus geographus. A set of overlapping cDNA clones were isolated and found to encode a Ca channel alpha-1 subunit, designated rbB-I. Polyclonal antiserum generated against a peptide from the rbB-I sequence selectively immunoprecipitates high-affinity I-125-labeled omega-conotoxin-binding sites from labeled rat forebrain membranes. PCR analysis shows that, like N-type Ca channels, expression of rbB-I is limited to the nervous system and neuronally derived cell lines. This brain Ca channel may mediate the omega-conotoxin-sensitive Ca influx required for neurotransmitter release at many synapses.