InterpretingResultsof Coulometryand Immunoprecipitationin DiagnosingIron Disorders
InterpretingResultsof Coulometryand Immunoprecipitationin DiagnosingIron Disorders
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库仑分析法和免疫沉淀法在诊断铁疾病中的解释结果
DOI：
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
C. Finch
中科院分区：
文献类型：
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作者：
Rettmer;Warren;C. Finch
血浆中铁的浓度、总铁结合力 (TIBC) 和转铁蛋白饱和度通常通过标准分光光度法测定，但铁浓度可以通过免疫沉淀法或电化学控制电位库仑法进行定量。由于这些铁测定法并不全部测量相同形式的铁，因此我们研究了处于不同铁营养状态的受试者：正常成人、缺铁患者、不饱和转铁蛋白或过饱和的地中海贫血患者转铁蛋白和特发性血色素沉着症患者。分光光度法和库仑法基本上检测到了血浆中所有的非原铁；结果相关性良好，但对库仑法显示出负面偏差。免疫沉淀程序的结果仅测量转铁蛋白结合的铁，与库仑法获得的结果相关性良好，但略高于后者。如果要准确诊断铁失调，临床实验室必须了解各种铁检测方法的特点。
Concentration of iron in plasma, total iron-binding capacity (TIBC), and transferrin saturation are often determined by standard spectrophotometric methods, but iron concentration may be quantified by immunoprecipitation or, electrochemically, by controlled-potential coulometry.Because theseiron assays do not all measure the same form(s) of iron, we studied subjects in various states of iron nutriture: normal adults, iron-deficient patients, thalassemia patients with unsaturated transfernn or oversaturated transferrin, and patients with idiopathic hemochromatosis. The spectrophotometric and coulometric methods detected essentially all nonhome iron in plasma; results correlated well but showed a negative bias toward the coulometric method. Results by an immunoprecipitation procedure, which measures only transferrin-bound iron, correlated well with those obtained coulometrically but were slightly higher than the latter. The characteristics of the various methods for iron must be understood bythe clinical Iabortory if diagnosis of iron disorders is to be accurate.
影响因子：
9.3
作者：
H. Huebers;M. Eng;B. Josephson;N. Ekpoom;R. Rettmer;R. Labbé;P. Pootrakul;C. Finch
通讯作者：
H. Huebers;M. Eng;B. Josephson;N. Ekpoom;R. Rettmer;R. Labbé;P. Pootrakul;C. Finch