Engineering microbes to sense and eradicate Pseudomonas aeruginosa, a human pathogen
Engineering microbes to sense and eradicate Pseudomonas aeruginosa, a human pathogen
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DOI：
10.1038/msb.2011.55
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发表时间：
2011-08-01
影响因子：
9.9
通讯作者：
Chang, Matthew Wook
中科院分区：
文献类型：
--
作者：
Saeidi, Nazanin;Wong, Choon Kit;Chang, Matthew Wook
合成生物学旨在系统地设计和构建新的生物系统，以解决能源，环境和健康问题。在本文中，我们描述了一种合成遗传系统的开发，该系统包括群体感应、杀伤和裂解装置，该系统使大肠杆菌能够通过绿脓菌素的产生和释放来感应和杀伤致病性铜绿假单胞菌菌株。对传感、杀伤和裂解装置进行表征，以阐明其检测、抗微生物和绿脓菌素释放功能，这随后有助于构建最终系统并验证其设计行为。我们证明了我们的工程E.大肠杆菌感测并杀死嗜酸性铜绿假单胞菌，活细胞减少99%证明了这一点。此外，我们还证明了我们设计的E.大肠杆菌抑制铜绿假单胞菌生物膜的形成接近90%，导致更稀疏和更薄的生物膜基质。这些结果表明，E.携带我们的合成遗传系统的大肠杆菌可能提供一种新的合成生物驱动的抗菌策略，可能适用于对抗铜绿假单胞菌和其他感染性病原体。Molecular Systems Biology 7：521; 2011年8月16日在线发表; doi：10.1038/msb.2011.55
Synthetic biology aims to systematically design and construct novel biological systems that address energy, environment, and health issues. Herein, we describe the development of a synthetic genetic system, which comprises quorum sensing, killing, and lysing devices, that enables Escherichia coli to sense and kill a pathogenic Pseudomonas aeruginosa strain through the production and release of pyocin. The sensing, killing, and lysing devices were characterized to elucidate their detection, antimicrobial and pyocin release functionalities, which subsequently aided in the construction of the final system and the verification of its designed behavior. We demonstrated that our engineered E. coli sensed and killed planktonic P. aeruginosa, evidenced by 99% reduction in the viable cells. Moreover, we showed that our engineered E. coli inhibited the formation of P. aeruginosa biofilm by close to 90%, leading to much sparser and thinner biofilm matrices. These results suggest that E. coli carrying our synthetic genetic system may provide a novel synthetic biology-driven antimicrobial strategy that could potentially be applied to fighting P. aeruginosa and other infectious pathogens. Molecular Systems Biology 7: 521; published online 16 August 2011; doi:10.1038/msb.2011.55