TRANSFER OF CHROMOSOMAL GENES AND PLASMIDS IN BACILLUS-THURINGIENSIS
TRANSFER OF CHROMOSOMAL GENES AND PLASMIDS IN BACILLUS-THURINGIENSIS
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DOI：
10.1128/aem.53.7.1525-1530.1987
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发表时间：
1987-07-01
影响因子：
4.4
通讯作者：
BECKMAN, W
中科院分区：
文献类型：
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作者：
ARONSON, AI;BECKMAN, W
通过细胞交配检测到从苏云金芽孢杆菌到蜡状芽孢杆菌的染色体基因转移频率很低，其中色氨酸标记是四个被测试基因中最频繁转移的基因。该过程对DNA酶具有抗性，且不受细胞滤液的调节。在供试的几个苏云金芽孢杆菌亚种中，以苏云金芽孢杆菌亚种的衍生物转移效果最好。Kurstaki HD1，它失去了几个质粒。所有的蜡状芽孢杆菌重组体都含有至少一个来自供体苏云金芽孢杆菌的质粒；通常，它是一个编码原毒素基因的质粒。在与苏云金杆菌亚种的交配中。库尔斯塔基HD1是一种2900万吨的质粒，含有与染色体同源的约2.5千碱基的区域，总是被转移。苏云金芽孢杆菌亚种中没有检测到染色体基因的转移。库尔斯塔克HD1菌株缺失该质粒，提示可能存在染色体动员。
A low frequency of chromosomal gene transfer from Bacillus thuringiensis to Bacillus cereus was detected by cell mating, with a tryptophan marker being the most frequently transferred gene among four that were tested. The process was resistant to DNase and was not mediated by cell filtrates. Among several B. thuringiensis subspecies tested, transfer was best with a derivative of B. thuringiensis subsp. kurstaki HD1, which lost several plasmids. All of the B. cereus recombinants contained at least one plasmid from the donor B. thuringiensis; frequently, it was a plasmid that encoded a protoxin gene. In matings with B. thuringiensis subsp. kurstaki HD1, a 29-megadalton plasmid that contained a ca. 2.5-kilobase region of homology with the chromosome was always transferred. No detectable transfer of chromosomal genes was found in B. thuringiensis subsp. kurstaki HD1 strains lacking this plasmid, suggesting that there may be chromosome mobilization.