Galvanotactic Migration of EA.Hy926 Endothelial Cells in a Novel Designed Electric Field Bioreactor
Galvanotactic Migration of EA.Hy926 Endothelial Cells in a Novel Designed Electric Field Bioreactor
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DOI：
10.1007/s12013-011-9231-3
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发表时间：
2011-12-01
影响因子：
2.6
通讯作者：
Wang, Zhengrong
中科院分区：
文献类型：
--
作者：
Long, Haiyan;Yang, Gang;Wang, Zhengrong
内源性直流电场（dcEF）在胚胎发生、伤口愈合和组织再生等主要生物过程中起着重要作用。本研究采用新型生物反应器对人脐静脉内皮细胞株EA.Hy926的趋电性进行了研究。采用数值模拟方法和等效电路模型方法对生物反应器的物理特性进行了讨论和分析。EA.Hy926细胞在生物反应器中在50 - 250 mV/mm dcEF下培养10 - 24 h。细胞迁移的方向，距离和速度记录在一个在线的延时显微镜。观察血清和生长因子对细胞迁移的影响。为了进一步探讨dcEFs对内皮细胞的调节作用，我们分析了生理强度的dcEFs对内皮细胞增殖和分泌一氧化氮（NO）、内皮素-1（ET-1）的影响。结果表明，EA.Hy926细胞具有明显的向阴极方向迁移的趋势，且这种迁移具有电压依赖性。dcEFs不影响细胞增殖，但影响NO和ET-1的产生。我们的研究还表明，新型生物反应器，具有紧凑和平面的风格，使它更方便，更合理的EF刺激实验比早期的腔室设计。
Endogenous direct current electric fields (dcEFs) play a significant role in major biological processes such as embryogenesis, wound healing, and tissue regeneration. In this study, the galvanotaxis of human umbilical vein endothelial cell line EA.Hy926 was investigated by using a novel designed bioreactor. The physical features of the bioreactor were discussed and analyzed by both numerical simulation method and equivalent circuit model method. EA.Hy926 cells were cultured in the bioreactor for 10-24 h under 50-250 mV/mm dcEFs. Cell migration direction, distance, and velocity were recorded under an online time-lapse microscope. The effects of serum and growth factor on cell galvanotatic migration were investigated. To further explore the role of dcEFs in regulating endothelial cells, we analyzed the endothelial cell proliferation and secretion of nitric oxide (NO), endothelin-1 (ET-1) in response to dcEFs of physiological strength. Our results showed that EA.Hy926 cells had an obvious directional migration to the cathode, and the EF-directed migration was voltage dependent. The results also showed dcEFs did not affect cell proliferation, but affected the productions of NO and ET-1. Our study also showed the novel bioreactor, with a compact and planar style, makes it more convenient and more reasonable for EF stimulation experiments than earlier chamber designs.