Retinoic acid signalling centres in the avian embryo identified by sites of expression of synthesising and catabolising enzymes
Retinoic acid signalling centres in the avian embryo identified by sites of expression of synthesising and catabolising enzymes
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DOI：
10.1002/dvdy.10292
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发表时间：
2003-05-01
影响因子：
2.5
通讯作者：
Maden, M
中科院分区：
文献类型：
--
作者：
Blentic, A;Gale, E;Maden, M
视黄酸是胚胎发育过程中的重要信号分子，但其在整个发育过程中的精确分布很难通过现有技术确定。通过使用原位杂交检查合成它的酶的分布是一种替代策略。在这里，我们描述了分布的三个维甲酸合成酶和维甲酸分解酶在鸡胚胎发生的早期阶段，目的是确定本地化的维甲酸信号区。涉及的酶是Raldh1、Raldh2、Raldh3和Cyp26A1。虽然这些分布已经被描述过，在这里，我们组装它们都在一个物种和几个新的酶表达的网站被确定，包括亨森氏结，心脏内胚层，推定胰腺内胚层，和背透镜。这项研究强调了控制视黄酸可用性的酶的动态表达模式以及视黄酸在整个胚胎发生过程中胚胎许多区域发育中的作用。这一策略为了解维甲酸畸形学和维甲酸缺乏综合征的表型提供了基础。
Retinoic acidis an important signalling molecule in the developing embryo, but its precise distribution throughout development is very difficult to determine by available techniques. Examining the distribution of the enzymes by which it is synthesised by using in situ hybridisation is an alternative strategy. Here, we describe the distribution of three retinoic acid synthesising enzymes and one retinoic acid catabolic enzyme during the early stages of chick embryogenesis with the intention of identifying localized retinoic acid signalling regions. The enzymes involved are Raldh1, Raldh2, Raldh3, and Cyp26A1. Although some of these distributions have been described before, here we assemble them all in one species and several novel sites of enzyme expression are identified, including Hensen's node, the cardiac endoderm, the presumptive pancreatic endoderm, and the dorsal lens. This study emphasizes the dynamic pattern of expression of the enzymes that control the availability of retinoic acid as well as the role that retinoic acid plays in the development of many regions of the embryo throughout embryogenesis. This strategy provides a basis for understanding the phenotypes of retinoic acid teratology and retinoic acid-deficiency syndromes.